CORE--BONE MORPHOMETRY AND MOLECULAR CYTOIMAGING

核心——骨形态学和分子细胞成像

• 批准号: 6316958
• 负责人: ROBERT Stewart WEINSTEIN
• 金额: $ 19.64万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316958
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical facility; bone density; bone marrow; histology; human tissue; in situ hybridization; information systems; photon absorptiometry; polymerase chain reaction; tissue /cell culture

The purpose of the Bone Morphometry and Molecular Cytoimaging Core is to consolidate key personnel and equipment to provide a centralized resource facility to enhance collaborative and multidisciplinary investigations into the cellular and molecular mechanisms of osteoporosis. The Core will perform three different procedures in a high-quality, reliable and cost- effective manner: bone densitometry, bone histomorphometry and histostaining of bone tissue and bone marrow cells combined with in situ hybridization or in situ use of the reverse transcriptase-polymerase chain reaction (RT-PCR). The measurement of longitudinal changes in murine bone mineral density by dual-energy X-ray absorptiometry (DXA or DEXA), a technique we have developed for this Program, is used to determine the differences in bone density in the senescence accelerated mouse (SAM) and its hybrid progeny, the changes in bone density with age, gonadectomy and treatment in thee mice and other strains and the optimal time for ex-vivo cell cultures and histomorphometric analysis of undecalcified bone specimens. The noninvasive and precise nature of DEXA supersedes more traditional methods for the estimation of murine bone magnitude such as ashing or cortical width morphometry. This core will also obtain, fix, embed, section, stain and quantify the bone histomorphometric features of the axial and appendicular skeleton of young and old mice for the needs of the various projects. In preliminary work leading to this application, it has become evident that the concurrent performance on adjacent bone sections of RT-PCR for specific messages and histostaining with cell markers such as tartrate resistant acid phosphatase (TRAP) for osteoclasts, nonspecific esterase (NSE) for macrophages or alkaline phosphatase (ALP) for osteoblasts combined with tetracycline-labeled bone histomorphometry is required to characterize distinct subsets of bone and bone marrow cells, their gene expression patterns and their activity. This core has done over 400 murine DEXA measurements in-vivo over the past six months (sharing use of a clinical densitometer on weekends from 8 A.M. to 10 P.M. or on weekdays after 5 P.M.) and has processed 85 human bone biopsies and over 60 murine specimens for histomorphometric examinations in the past year. The bone laboratory is a CAP accredited facility with the ability to safely archive and store tissue and data. Bone densitometry and histomorphometric results will be appended to a relational database for prompt return to the principal investigators of each project.

骨形态测量和分子细胞成像核心的目的是为了 整合关键人员和设备，提供集中资源 加强协作和多学科调查的设施 骨质疏松症的细胞和分子机制。核心意志 以高质量、可靠和成本的方式执行三种不同的程序- 有效方式：骨密度测量、骨组织形态计量学 骨组织和骨髓细胞的组织化学染色与原位结合 逆转录聚合酶链的杂交或原位使用 反应(RT-PCR)。小鼠骨骼纵向变化的测量 用双能X射线吸收法(DXA或DEXA)测定矿物密度，a 我们为本计划开发的技术，用于确定 加速衰老小鼠(SAM)和衰老小鼠(SAM)骨密度的差异 它的杂交后代，骨密度随年龄的变化，性腺切除和 小鼠和其他品系的治疗及体外最适时间 不脱钙骨的细胞培养及组织形态计量学分析 标本。DEXA的非侵入性和精确度可以取代更多 小鼠骨骼大小的传统估计方法，如 灰化或皮质宽度形态测量。这个核心还将获得、修复、 骨组织形态计量学特征的包埋、切片、染色和量化 幼龄和老年小鼠的轴向和附肢骨骼 各种项目。在这一应用程序的前期工作中，它 已经很明显，相邻骨骼上的并行性能 特定信息的RT-PCR切片及细胞组织染色 抗酒石酸酸性磷酸酶(TRAP)等标志物 破骨细胞，巨噬细胞或碱性细胞的非特异性酯酶(NSE) 成骨细胞与四环素标记骨结合的磷酸酶 需要组织形态计量学来表征不同的骨亚组和 骨髓细胞、其基因表达模式及其活性。 在过去的时间里，这个核心已经在体内进行了400多次小鼠的DEXA测量 六个月(从上午8点开始在周末共享临床密度计) 至晚上10时或平日下午5时后)已经加工了85块人骨 组织形态计量学检查的活组织切片和60多个小鼠标本 在过去的一年。骨骼实验室是CAP认证的机构，具有 能够安全地归档和存储组织和数据。骨 密度计量学和组织形态计量学结果将附加到 用于迅速返回给主要调查员的关系数据库 每个项目。