BONE MORPHOMETRY AND BIOMECHANICS CORE

骨形态学和生物力学核心

• 批准号: 7094993
• 负责人: ROBERT Stewart WEINSTEIN
• 金额: $ 34.68万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7094993
• 关键词: aging; beta galactosidase; biomechanics; biomedical facility; bone density; bone imaging /visualization /scanning; cellular pathology; compression; computed axial tomography; enzyme activity; genetically modified animals; histology; human tissue; laboratory mouse; mechanical stress; molecular pathology; morphometry; osteoblasts; osteoclasts; osteoporosis; photon absorptiometry; terminal nick end labeling; training

The purpose of this core is to consolidate key personnel and equipment to provide a centralized facility to enhance collaborative and multidisciplinary investigations into the multiple mechanisms of osteoporosis in old age. Core C will perform six labor-intensive and highly specialized procedures in a cost- and timeefficient manner to facilitate the examination of the changes in bone cells, architecture, mass, and strength that accompany old age. Aim 1. Prepare undecalcified, plastic-embedded, tetracycline-labeled bone sections to supply the project leaders with the materials necessary for a quantitative static and dynamic histological analysis of axial (lumbar vertebrae) and appendicular (distal femoral) bone. The core will also prepare sections by in-situ nick-end labeling to determine the prevalence of osteoblast and osteocyte apoptosis. Aim 2. Obtain frozen sections of undecalcified bone using a dedicated cryostat and tape-transfer system to facilitate quantification of p-galactosidase activity, immunostaining of targeted cells in transgenic animals, and recognition of green fluorescent protein at the same time as tetracycline labels. These needs of the program cannot be accomplished with standard plastic-embedded or decalcified, paraffin-embedded sections. The core will also be responsible for freezer storage of these specimens. Aim 3. Perform micro-CT measurements using a unique horizontal imaging system to acquire images comparable to those obtained by longitudinal sections for bone histology. In a customized specimen holder that accommodates a stack of 10 bones, lumbar vertebrae or femora will be scanned to determine the individual volumetric densitometric contributions of cortical and cancellous bone. In addition, three-dimensional measurements of cortical and cancellous microarchitecture will be done. Aim 4. Provide murine bone mass determinations by dual-energy x-ray absorptiometry on Hologic or PIXImus instruments to meet the needs of the project leaders. Measurements of global, spinal, and femoral bone density will be performed serially, in longitudinal experiments, in live mice. Aim 5. Acquire biomechanical measurements of murine vertebral compression strength and femoral three-point bending using a material testing system to determine the significance of changes in bone mineral density and bone architecture. Aim 6. Train key personnel from the projects that need bone mass, biomechanical and histomorphometric measurements to help each team meet the goal of their projects. This centralized facility will provide for each of the projects a uniform and comprehensive investigation of the skeletal changes in bone mass, architecture, turnover and strength that occur with aging allowing determination of the efficacy of different treatment regimens in preventing fragility fractures.

该核心的目的是整合关键人员和设备，提供集中设施， 加强对骨质疏松症多种机制的合作和多学科研究， 老年。核心C将执行六个劳动密集型和高度专业化的程序， 有助于检查骨细胞、结构、质量和强度变化的方式 伴随着老年。目标1.制备不脱钙、塑料包埋、四环素标记的骨 各部门为项目负责人提供必要的材料， 轴骨（腰椎）和无骨（股骨远端）组织学分析。核心还将 通过原位缺口末端标记制备切片，以确定成骨细胞和骨细胞的存在率 凋亡目标2.使用专用低温恒温器和胶带转移获得未脱钙骨的冷冻切片 促进β-半乳糖苷酶活性定量、转基因细胞中靶细胞免疫染色的系统 动物，并在四环素标记的同时识别绿色荧光蛋白。这些需求 该计划不能完成与标准的塑料包埋或脱钙，石蜡包埋 路段核心还将负责这些标本的冷冻储存。目标3.执行微型CT 使用独特的水平成像系统进行测量，以获取与所获得的图像相当的图像。 纵向切片进行骨组织学检查在容纳一叠样品的定制样品保持器中， 将扫描10块骨骼、腰椎或股骨，以确定个体体积密度 皮质骨和松质骨的贡献。此外，皮质和皮质骨的三维测量 将完成松质骨微结构。目标4。通过双能量法测定小鼠骨量 在Hologic或PIXImus仪器上进行X射线吸收测量，以满足项目负责人的需求。 整体、脊柱和股骨骨密度的测量将连续进行，纵向 实验，在活老鼠身上。目标5。获取小鼠椎体压缩的生物力学测量值 强度和股骨三点弯曲，使用材料测试系统来确定 骨矿物质密度和骨结构的变化。目标6。培训项目的关键人员， 需要骨量，生物力学和组织形态测量，以帮助每个团队达到目标， 他们的项目。这个集中的设施将为每个项目提供统一和全面的 研究随着年龄增长发生的骨量、结构、转换和强度的骨骼变化 允许确定不同治疗方案在预防脆性骨折中的功效。