BONE MORPHOMETRY AND BIOMECHANICS CORE
骨形态学和生物力学核心
基本信息
- 批准号:7094993
- 负责人:
- 金额:$ 34.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-06-01 至 2011-05-31
- 项目状态:已结题
- 来源:
- 关键词:agingbeta galactosidasebiomechanicsbiomedical facilitybone densitybone imaging /visualization /scanningcellular pathologycompressioncomputed axial tomographyenzyme activitygenetically modified animalshistologyhuman tissuelaboratory mousemechanical stressmolecular pathologymorphometryosteoblastsosteoclastsosteoporosisphoton absorptiometryterminal nick end labelingtraining
项目摘要
The purpose of this core is to consolidate key personnel and equipment to provide a centralized facility to
enhance collaborative and multidisciplinary investigations into the multiple mechanisms of osteoporosis in
old age. Core C will perform six labor-intensive and highly specialized procedures in a cost- and timeefficient
manner to facilitate the examination of the changes in bone cells, architecture, mass, and strength
that accompany old age. Aim 1. Prepare undecalcified, plastic-embedded, tetracycline-labeled bone
sections to supply the project leaders with the materials necessary for a quantitative static and dynamic
histological analysis of axial (lumbar vertebrae) and appendicular (distal femoral) bone. The core will also
prepare sections by in-situ nick-end labeling to determine the prevalence of osteoblast and osteocyte
apoptosis. Aim 2. Obtain frozen sections of undecalcified bone using a dedicated cryostat and tape-transfer
system to facilitate quantification of p-galactosidase activity, immunostaining of targeted cells in transgenic
animals, and recognition of green fluorescent protein at the same time as tetracycline labels. These needs
of the program cannot be accomplished with standard plastic-embedded or decalcified, paraffin-embedded
sections. The core will also be responsible for freezer storage of these specimens. Aim 3. Perform micro-CT
measurements using a unique horizontal imaging system to acquire images comparable to those obtained
by longitudinal sections for bone histology. In a customized specimen holder that accommodates a stack of
10 bones, lumbar vertebrae or femora will be scanned to determine the individual volumetric densitometric
contributions of cortical and cancellous bone. In addition, three-dimensional measurements of cortical and
cancellous microarchitecture will be done. Aim 4. Provide murine bone mass determinations by dual-energy
x-ray absorptiometry on Hologic or PIXImus instruments to meet the needs of the project leaders.
Measurements of global, spinal, and femoral bone density will be performed serially, in longitudinal
experiments, in live mice. Aim 5. Acquire biomechanical measurements of murine vertebral compression
strength and femoral three-point bending using a material testing system to determine the significance of
changes in bone mineral density and bone architecture. Aim 6. Train key personnel from the projects that
need bone mass, biomechanical and histomorphometric measurements to help each team meet the goal of
their projects. This centralized facility will provide for each of the projects a uniform and comprehensive
investigation of the skeletal changes in bone mass, architecture, turnover and strength that occur with aging
allowing determination of the efficacy of different treatment regimens in preventing fragility fractures.
这一核心的目的是巩固关键人员和设备,提供一个集中的设施
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ROBERT Stewart WEINSTEIN其他文献
ROBERT Stewart WEINSTEIN的其他文献
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{{ truncateString('ROBERT Stewart WEINSTEIN', 18)}}的其他基金
Glucocorticoids, Bone Strength and Angiogenesis
糖皮质激素、骨强度和血管生成
- 批准号:
7785356 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoid-induced osteonecrosis of the hip, osteocytes and canalicular fluid
糖皮质激素引起的髋部、骨细胞和小管液的骨坏死
- 批准号:
8974246 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoid-induced osteonecrosis of the hip, osteocytes and canalicular fluid
糖皮质激素引起的髋部、骨细胞和小管液的骨坏死
- 批准号:
9339480 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoids, Bone Strength and Angiogenesis
糖皮质激素、骨强度和血管生成
- 批准号:
8391151 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoid-induced osteonecrosis of the hip, osteocytes and canalicular fluid
糖皮质激素引起的髋部、骨细胞和小管液的骨坏死
- 批准号:
8912853 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoids, Bone Strength and Angiogenesis
糖皮质激素、骨强度和血管生成
- 批准号:
8195624 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoid-induced osteonecrosis of the hip, osteocytes and canalicular fluid
糖皮质激素引起的髋部、骨细胞和小管液的骨坏死
- 批准号:
8738790 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
Glucocorticoids, Bone Strength and Angiogenesis
糖皮质激素、骨强度和血管生成
- 批准号:
7914245 - 财政年份:2009
- 资助金额:
$ 34.68万 - 项目类别:
GLUOCORTICOIDS, OSTEOCYTES, BONE STENGTH IN AGE-RELATED
糖皮质激素、骨细胞、年龄相关的骨强度
- 批准号:
7094998 - 财政年份:2006
- 资助金额:
$ 34.68万 - 项目类别:
CORE--BONE MORPHOMETRY AND MOLECULAR CYTOIMAGING
核心——骨形态学和分子细胞成像
- 批准号:
6316958 - 财政年份:2000
- 资助金额:
$ 34.68万 - 项目类别:
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