CORE--NEUROPATHOLOGY AND BRAIN BANK

核心——神经病理学和脑库

• 批准号: 6299227
• 负责人: Daniel P Perl
• 金额: $ 21.2万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299227
• 关键词: Alzheimer's disease; biomedical facility; brain; brain disorder diagnosis; human tissue; neuropathology; postmortem; tissue resource /registry

The specific aims of the Brain Bank Core are to obtain, characterize, describe, dissect, preserve and distribute to different researchers engaged in Alzheimer's disease research, brain tissues obtained from normal control, and Alzheimer's disease subjects. The Brain Bank Core will obtain and bank brain tissues from as many of the subjects enrolled by the Recruitment and Diagnosis Core and studied by the Neurophyschology project as possible. The Brain Bank Core will perform autopsies with the lease possible post-mortem delay, and will maintain permanently all pertinent specimen related information in a manner accessible to all past, present and future investigators using the Brain Bank. The acquisition and banking of the projected cohorts will provide a unique resource to only for the proposed studies, but for generations of studies and investigators to come. The brain banking procedures outlined in this application have been designed to preserved the tissues optimally for as broad a set of experimental methodologies as possible. After extraction, brain specimens will be photographed, weighted and any gross lesions and abnormalities note. The right half of the brain will be preserved in paraformaldehyde for neuropathological and neuroanatomical studies. The left half will be dissected in 0.5-0.8 cm coronal slabs, and after identification of the structures in each slab they will be snap frozen in liquid freon. Frozen slabs will be kept at -80C until sub-dissected for use by specific studies. The Brain Bank Core will serve all five projects proposed in this application either directly or indirectly. The Brain Bank Core will contribute to the Neuropsychology, Family History and Acute Phase Reactants projects (Projects 1, 2 and 4) by performing autopsies of subjects who have been studied in life. The neuropathological information obtained (Project 5) on these cases will enhance the Neuropsychology project by providing data on the neuropathological of cognitive decline in the aged. The Neurochemistry project (Project 3) will use the tissues banked by this core to investigate the neurochemical correlates of cognitive decline in the aged. The procedures used by the Brain Bank Core have been optimized to enhance and facilitate multiple neurochemical studies of each case banked. In addition the acquisition and banking of brain tissues from this unique cohort of subjects will augment the utility of the Brain Bank for projects funded by other grants (eg., ADRC, Cellular and Molecular Markers in Schizophrenia-PPG), by providing specimens from normal elderly controls whose cognitive status has been confirmed by rigorous ante- mortem testing.

脑库核心的具体目的是获得，表征， 描述，剖析，保存和分发给不同的研究人员 从事阿尔茨海默氏病研究，从 正常控制和阿尔茨海默氏病。 脑库核心 将从招收的许多受试者中获取并养活脑组织 通过招募和诊断核心，并通过神经生理学进行研究 尽可能项目。 脑库核心将通过 租赁可能的后延迟，并将永久维护所有 所有人都可以访问的方式相关的标本相关信息 过去，现在和未来的调查人员使用脑库。 这 收购和对预计队列的银行业将提供独特的 资源仅用于拟议的研究，但几代研究 和调查人员来了。 大脑银行程序概述了 应用已设计为最佳地保存组织 尽可能宽阔一组实验方法。 提取后， 脑标本将被拍照，加权和任何总病变， 异常注意。 右半大脑将被保存在 用于神经病理学和神经解剖学研究的多聚甲醛。 这 左半将在0.5-0.8 cm的冠状板中解剖，之后 识别每个平板中的结构，它们将被冻结 在液体弗莱恩。 冷冻平板将保持在-80℃直至细分 用于特定研究。 脑库核心将为所有五个 直接或间接地在本应用程序中提出的项目。 这 脑库核心将有助于神经心理学，家族史 和急性相应物项目（项目1、2和4）通过执行 在生活中研究的受试者的尸检。 这 在这些情况下获得的神经病理学信息（项目5）将 通过提供有关的数据来增强神经心理学项目 老年认知能力下降的神经病理学。 神经化学 项目（项目3）将使用该核心依据的组织 研究认知下降的神经化学相关性 老化。 脑库核心使用的程序已被优化为 增强和促进每种情况的多次神经化学研究 银行。 另外，从 这个独特的主题队列将增加大脑库的实用性 对于其他赠款资助的项目（例如，ADRC，细胞和分子 精神分裂症-PPG的标记），通过提供正常老年人的标本 严格的前动物证实了认知状况的对照 验尸测试。