CORE--NUEROPATHOLOGY /MOLECULAR

核心--神经病理学/分子

• 批准号: 6299271
• 负责人: JAMES GOLDMAN
• 金额: $ 32.23万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299271
• 关键词: Alzheimer's disease; DNA; apolipoprotein E; biomedical facility; brain disorder diagnosis; genotype; human genetic material tag; human tissue; neuropathology; postmortem; tissue resource /registry

The Pathology-Molecular Core for this project will continue to provide postmortem diagnosis and will now provide apolipoprotein-E genotypes and storage capacity for serum and plasma for the entire cohort. The primary purpose of this core has been to support the clinical projects by providing state of the art neuropathological diagnosis. Because the program project was, from its inception, designed to be a large-scale epidemiologic project, routine or mandatory brain autopsy was not a realistic criterion for inclusion into the studies. However, we now intend to provide educational seminars in the community with the hope of enhancing autopsy acquisition. Our banked tissue has also been used for several studies examining the relationship between Alzheimer's disease (AD) and APOE and in a collaborative study of APOE in the diagnosis of AD. The importance of having autopsy confirmed diagnoses is obvious, and now, we will make a concerted effort to ascertain autopsy in patients at any stge of disease and controls. Because this project has made great strides in community relations regarding autopsy we are confident that the rate of autopsy confirmation will improve. Preliminary data indicates with efforts focused on the families of patients with late-stage disease, the rate of acceptance of autopsy can approach 50%. In addition to providing a neuropathological examination of participation who die in the program project, we will complete data entry forms for entry into program project database, collect and store fresh blood samples as serum and plasma for planned investigations, extract and store DNA for APOE genotypes and other planned genetic studies, distribute additional serum, plasma or DNA as needed to study investigators, provide relevant tissues to complete aims in projects related to APOE expression and interact with Columbia University's Taub Alzheimer's Disease Center Research Center.

该项目的病理学-分子核心将继续提供尸检诊断，现在将提供整个队列的血清和血浆的载脂蛋白E基因型和储存能力。该核心的主要目的是通过提供最先进的神经病理学诊断来支持临床项目。由于该项目从一开始就被设计为一个大规模的流行病学项目，因此常规或强制性的脑尸检并不是纳入研究的现实标准。不过，我们现在打算在社区举办教育研讨会，希望能增加尸检的采购。我们的库存组织也已被用于几项研究，检查阿尔茨海默病（AD）和载脂蛋白E之间的关系，并在诊断AD的载脂蛋白E的合作研究。尸检确认诊断的重要性是显而易见的，现在，我们将共同努力，以确定在疾病和控制的任何阶段的患者尸检。因为这个项目在尸检方面的社区关系方面取得了很大的进步，我们相信尸检确认率将会提高。初步数据显示，在对晚期疾病患者家属的关注下，尸检的接受率可接近50%。除了为在项目中死亡的参与者提供神经病理学检查外，我们还将填写数据录入表以录入项目数据库，收集并储存新鲜血液样本作为血清和血浆用于计划的研究，提取并储存DNA用于APOE基因型和其他计划的遗传研究，根据需要向研究者分发额外的血清、血浆或DNA，提供相关组织以完成与APOE表达相关的项目，并与哥伦比亚大学的Taub阿尔茨海默病中心研究中心进行互动。