CORE--NUEROPATHOLOGY /MOLECULAR

该项目的病理分子核心将继续提供死后诊断，现在将为整个队列提供载脂蛋白e基因型和血清和血浆的储存能力。这个核心的主要目的是通过提供最先进的神经病理诊断来支持临床项目。由于该项目从一开始就被设计为一个大规模的流行病学项目，常规或强制性的脑尸检并不是纳入研究的现实标准。然而，我们现在打算在社区提供教育研讨会，希望加强尸体解剖的获取。我们储存的组织也被用于几项研究阿尔茨海默病（AD）和APOE之间关系的研究，以及APOE在AD诊断中的合作研究。尸检确认诊断的重要性是显而易见的，现在，我们将共同努力，确定在任何阶段的疾病和控制的患者尸检。由于该项目在尸检方面的社区关系方面取得了很大进展，我们相信尸检确认率将会提高。初步数据表明，随着对晚期疾病患者家属的努力，尸检的接受率可以接近50%。除了为在项目中死亡的参与者提供神经病理学检查外，我们还将完成数据输入表格以进入项目数据库，收集和储存新鲜血液样本作为血清和血浆用于计划调查，提取和储存DNA用于APOE基因型和其他计划的遗传研究，根据需要分发额外的血清、血浆或DNA给研究人员。为完成APOE表达相关项目提供相关组织，并与哥伦比亚大学陶布阿尔茨海默病研究中心相互作用。

The Pathology-Molecular Core for this project will continue to provide postmortem diagnosis and will now provide apolipoprotein-E genotypes and storage capacity for serum and plasma for the entire cohort. The primary purpose of this core has been to support the clinical projects by providing state of the art neuropathological diagnosis. Because the program project was, from its inception, designed to be a large-scale epidemiologic project, routine or mandatory brain autopsy was not a realistic criterion for inclusion into the studies. However, we now intend to provide educational seminars in the community with the hope of enhancing autopsy acquisition. Our banked tissue has also been used for several studies examining the relationship between Alzheimer's disease (AD) and APOE and in a collaborative study of APOE in the diagnosis of AD. The importance of having autopsy confirmed diagnoses is obvious, and now, we will make a concerted effort to ascertain autopsy in patients at any stge of disease and controls. Because this project has made great strides in community relations regarding autopsy we are confident that the rate of autopsy confirmation will improve. Preliminary data indicates with efforts focused on the families of patients with late-stage disease, the rate of acceptance of autopsy can approach 50%. In addition to providing a neuropathological examination of participation who die in the program project, we will complete data entry forms for entry into program project database, collect and store fresh blood samples as serum and plasma for planned investigations, extract and store DNA for APOE genotypes and other planned genetic studies, distribute additional serum, plasma or DNA as needed to study investigators, provide relevant tissues to complete aims in projects related to APOE expression and interact with Columbia University's Taub Alzheimer's Disease Center Research Center.