A massively parallel DNA sequencer

描述(由申请人提供)：我们请求500,000美元购买大规模并行DNA测序仪，供哥伦比亚大学社区和其他机构的合作者使用。在哥伦比亚大学的任何一个校区都没有类似的仪器。在这台仪器上进行一次测序，将相当于在哥伦比亚大学目前可用的两台仪器上分别进行100多次测序，从而极大地扩大了研究计划的范围。 这一工具将被广泛的NIH资助的调查人员使用。项目范围广泛，从微生物比较基因组学到确定与不同形式的致病性相关的基因和基因组区域，流感病毒感染对细菌菌群影响的元基因组学研究，参与剪接、转录和组织特异性miRNA物种的全球基础研究，以及定向酶进化中的序列群体；预计的许多用途将涉及确定从肾小球肾炎到糖尿病、神经精神疾病到脱发、淋巴瘤到心律失常等广泛疾病的基因变化，预计结果将有助于深入了解疾病的机制、诊断、预后和治疗。 该仪器设在基因组中心，将由在DNA测序和方法开发方面经验丰富的工作人员操作，并得到所有必要的辅助仪器和计算资源的支持。哥伦比亚大学承诺为延长保修期提供支持，并在第一年半的运行期间配备一名技术人员，以表明其对该仪器的维护和操作的承诺，之后该仪器将由用户费用提供支持。鉴于哥伦比亚大学校园里有大量的生物医学研究人员，这种仪器的存在将对NIH资助的研究产生重大影响。

DESCRIPTION (provided by applicant): We are requesting $500,000 to purchase a massively parallel DNA sequencer for use by the Columbia University community and collaborators at other institutions. There is no comparable instrument available on any of the campuses of Columbia University. A single sequencing run on this instrument will achieve the equivalent of over 100 sequencing runs on each of Columbia University's currently available 2 instruments, thus dramatically expanding the scope of research programs. This instrument will be used by a broad range of NIH-funded investigators. Projects range from comparative genomics of microorganisms to identify genes and genome regions associated with distinct forms of pathogenicity, a metagenomics study of the effect of influenza virus infection on bacterial flora, global basic research studies of motifs involved in splicing, transcription, and of tissue specific miRNA species, and populations of sequences in directed enzyme evolution; much of the projected use will involve identification of genetic changes involved in a wide range of diseases from glomerulonephritis to diabetes, neuropsychiatric disorders to alopecia, lymphoma to arrhythmias, with results anticipated to provide insight into the mechanism of disease, diagnosis, prognosis and treatment. The instrument, housed in the Genome Center, will be run by staff well experienced with DNA sequencing and methods development, and supported by all required ancillary instruments and computational resources. Columbia University has demonstrated its commitment to the maintenance and operation of this instrument by pledging support for warranty extension and a technician covering the first year and a half of operation, following which the instrument will be supported by user fees. Given the large number of biomedical investigators on the campuses of Columbia University, the presence of such an instrument would have a significant impact on NIH-funded research.