Simultaneous Near-Field and Confocal Imaging

同时近场和共焦成像

• 批准号: 6404297
• 负责人: Michael J Szulczewski
• 金额: $ 14.61万
• 依托单位: PRAIRIE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-20 至 2002-03-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6404297
• 关键词: astrocytes; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; computer system design /evaluation; computer system hardware; confocal scanning microscopy; fluorescence microscopy; fluorescence polarization; image enhancement; imaging /visualization /scanning; ion transport; laboratory mouse; lasers; light scattering; mitochondria; nanotechnology; neurons; synaptic vesicles; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant): Recent developments in near-field instrumentation have provided PRAIRIE TECHNOLOGIES with the necessary understanding to develop a multi-mode confocal microscope that will integrate confocal microscopy with simultaneous high-speed fluorescence measurements as well as sub-diffraction resolution imaging. This powerful new tool will revolutionize scientists' ability to study neuronal function and dysfunction in living systems. In phase I we will build and test an alpha prototype by integrating a high-speed fluorescence detection path into our existing confocal microscope. In phase II, we will use the knowledge gained in this phase I feasibility to design and build a commercial simultaneous near-field and confocal imaging system. The resultant product will permit the scientist to address significant questions concerning ion fluxes, synaptic transmission and the molecular nature of learning and memory. PROPOSED COMMERCIAL APPLICATION: Not Available

描述(由申请人提供)：近场技术的最新发展 仪器设备为Prairie Technologies提供了必要的 对研制集成化多模共焦显微镜的认识 具有同步高速荧光测量的共聚焦显微镜 以及亚衍射分辨率成像。这款功能强大的新工具将 使科学家研究神经元功能和功能障碍的能力发生革命性变化 生命系统。在第一阶段中，我们将通过以下方式构建和测试阿尔法原型 将高速荧光检测路径集成到我们现有的共焦中 显微镜。在第二阶段，我们将使用在第一阶段中获得的知识 设计和建造商用同步近场和 共焦成像系统。 由此产生的产品将使科学家能够解决重要的 关于离子通量、突触传递和分子性质的问题 学习和记忆的能力。 建议的商业应用： 不可用