Simultaneous Near-Field and Confocal Imaging

同时近场和共焦成像

• 批准号: 6404297
• 负责人: Michael J Szulczewski
• 金额: $ 14.61万
• 依托单位: PRAIRIE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-20 至 2002-03-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6404297
• 关键词: astrocytes; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; computer system design /evaluation; computer system hardware; confocal scanning microscopy; fluorescence microscopy; fluorescence polarization; image enhancement; imaging /visualization /scanning; ion transport; laboratory mouse; lasers; light scattering; mitochondria; nanotechnology; neurons; synaptic vesicles; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant): Recent developments in near-field instrumentation have provided PRAIRIE TECHNOLOGIES with the necessary understanding to develop a multi-mode confocal microscope that will integrate confocal microscopy with simultaneous high-speed fluorescence measurements as well as sub-diffraction resolution imaging. This powerful new tool will revolutionize scientists' ability to study neuronal function and dysfunction in living systems. In phase I we will build and test an alpha prototype by integrating a high-speed fluorescence detection path into our existing confocal microscope. In phase II, we will use the knowledge gained in this phase I feasibility to design and build a commercial simultaneous near-field and confocal imaging system. The resultant product will permit the scientist to address significant questions concerning ion fluxes, synaptic transmission and the molecular nature of learning and memory. PROPOSED COMMERCIAL APPLICATION: Not Available

描述（由申请人提供）：近场技术的最新发展 仪器设备为PRAIRIE EQUIPLOGIES提供了必要的 了解开发一个多模式共聚焦显微镜，将集成 共聚焦显微镜与同步高速荧光测量， 以及亚衍射分辨率成像。这个强大的新工具将 彻底改变了科学家研究神经元功能和功能障碍的能力， 生活系统在第一阶段，我们将建立和测试一个阿尔法原型， 将高速荧光检测路径集成到我们现有的共聚焦 显微镜在第二阶段，我们将利用在第一阶段获得的知识 设计和建造商业同时近场和 共焦成像系统 由此产生的产品将允许科学家解决重大问题， 关于离子流、突触传递和分子性质的问题 学习和记忆。 拟定商业应用： 不可用