Integrated Twin Epi-Flourescence System

集成双落射荧光系统

• 批准号: 6402386
• 负责人: Michael J Szulczewski
• 金额: $ 14.01万
• 依托单位: PRAIRIE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2002-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6402386
• 关键词: biomedical equipment development; calcium; clathrate; digital imaging; fluorescence microscopy; hippocampus; laboratory rat; learning; memory; neural transmission; neuroimaging; optics; photolysis; sectioning; tissue /cell culture

DESCRIPTION (provided by applicant): Cellular studies of learning and memory, and synaptic transmission are significantly hampered by standard optical configurations that were developed decades ago. This is especially true when considering present day, fluorescence microscope technology where a single optical input pathway provides wide-field illumination. For example, the integration of ion imaging with UV photolysis is compromised since focal UV excitation must be forced into the optical pathway that was designed only for epi-fluorescence illumination. The goal of this project is to test the feasibility of developing a twin-illumination pathway for fluorescencemicroscopes to allow the versatility necessary for the experiments of today and tomorrow. We will: 1) Design and build a twin-illumination pathway that will integrate with present day upright microscopes. 2) Test the optical properties of each illumination pathway for flatness of field, full-field illumination and light transmission properties. 3) Determine the reliability of motor control for positioning apertures and a UV transmitting optical fiber coupling mechanism in one of the illumination pathways. 4) Test and evaluate the feasibility of using "pass through optics to monitor the location of laser illumination beams for photolysis. 5) Test the twin-illumination system in photolysis experiments and in studies to monitor fast calcium transients. PROPOSED COMMERCIAL APPLICATION: This twin-IIumination system has many potential applications in science: simultaneous imaging together with focal ablation or photolysis, and by including apertures in one arm of the excitation pathway, it will be possible to use one pathway for restricted field excitation for measurement of fast changes in calcium in neuronal processes.

描述（由申请人提供）：学习和记忆的细胞研究， 和突触传播受标准光学显着阻碍 几十年前开发的配置。尤其如此 考虑到今天，荧光显微镜技术 光学输入途径可提供宽场照明。例如， 离子成像与紫外光解的整合受到损害，因为焦图 兴奋必须被迫进入仅设计的光通路 荧光照明。该项目的目的是测试 开发双胞胎弹性途径的可行性 荧光大体镜可以实验所需的多功能性 今天和明天。我们将：1）设计和建造双胞胎弹性途径 这将与当今的直立显微镜集成。 2）测试光学 每个照明途径的特性 照明和光传输特性。 3）确定可靠性 用于定位光圈和紫外线传输光纤的电动机控制 一条照明途径中的耦合机制。 4）测试和评估 使用“通过光学来监视激光位置的可行性 照明梁用于光解。 5）测试双胞胎刷新系统 光解实验和研究以监测快速钙瞬变。 拟议的商业应用： 这个双二氮化系统在科学中具有许多潜在的应用：同时成像与局灶性消融或光解有关，并且通过在激发途径的一个部门中包括孔，可以使用一种途径来限制野外激发，以测量神经元过程中钙快速变化的测量。