Integrated Twin Epi-Flourescence System

集成双落射荧光系统

• 批准号: 6402386
• 负责人: Michael J Szulczewski
• 金额: $ 14.01万
• 依托单位: PRAIRIE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2002-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6402386
• 关键词: biomedical equipment development; calcium; clathrate; digital imaging; fluorescence microscopy; hippocampus; laboratory rat; learning; memory; neural transmission; neuroimaging; optics; photolysis; sectioning; tissue /cell culture

DESCRIPTION (provided by applicant): Cellular studies of learning and memory, and synaptic transmission are significantly hampered by standard optical configurations that were developed decades ago. This is especially true when considering present day, fluorescence microscope technology where a single optical input pathway provides wide-field illumination. For example, the integration of ion imaging with UV photolysis is compromised since focal UV excitation must be forced into the optical pathway that was designed only for epi-fluorescence illumination. The goal of this project is to test the feasibility of developing a twin-illumination pathway for fluorescencemicroscopes to allow the versatility necessary for the experiments of today and tomorrow. We will: 1) Design and build a twin-illumination pathway that will integrate with present day upright microscopes. 2) Test the optical properties of each illumination pathway for flatness of field, full-field illumination and light transmission properties. 3) Determine the reliability of motor control for positioning apertures and a UV transmitting optical fiber coupling mechanism in one of the illumination pathways. 4) Test and evaluate the feasibility of using "pass through optics to monitor the location of laser illumination beams for photolysis. 5) Test the twin-illumination system in photolysis experiments and in studies to monitor fast calcium transients. PROPOSED COMMERCIAL APPLICATION: This twin-IIumination system has many potential applications in science: simultaneous imaging together with focal ablation or photolysis, and by including apertures in one arm of the excitation pathway, it will be possible to use one pathway for restricted field excitation for measurement of fast changes in calcium in neuronal processes.

描述（由申请人提供）：学习和记忆的细胞研究， 和突触传递受到标准光学的严重阻碍 几十年前开发的配置。当 考虑到当今，荧光显微镜技术只需一个 光学输入路径提供宽视场照明。例如， 由于聚焦紫外光，离子成像与紫外光解的集成受到影响 激发必须强制进入光路，该光路仅设计用于 落射荧光照明。该项目的目标是测试 开发双照明路径的可行性 荧光显微镜可实现实验所需的多功能性 今天和明天。我们将：1）设计并建造双照明路径 它将与当今的正置显微镜集成。 2）测试光学 每个照明路径的场平坦度、全场特性 照明和光传输特性。 3）确定可靠性 用于定位孔径和紫外线传输光纤的电机控制 照明路径之一中的耦合机制。 4）测试和评估 利用“透过光学”监测激光位置的可行性 用于光解的照明光束。 5) 测试双照明系统 光解实验和监测快速钙瞬变的研究。 拟议的商业应用： 这种双照明系统在科学上有许多潜在的应用：同时成像与焦点消融或光解，并且通过在激发通路的一个臂中包含孔径，将有可能使用限制场激发的一个通路来测量神经元过程中钙的快速变化。