The Swept Field Confocal Microscope

描述(由申请人提供) 共焦显微镜是生物科学的重要工具。它有 大大加强了神经科学中假说的测试，并已 提供了对神经性疾病的原因的见解。这样做的目的是 项目是基于一种新的概念开发一种新型的共焦显微镜， 扫场共焦设计(SFC)香港证监会有潜力成为 个人可以购买的光学显微镜附件 科学家的工资不到5万美元。证监会是一项革命性的联合焦点概念， 这种混合仪器使用86x12的固定针孔阵列 在使用一个轴(例如Y轴)的同时沿一个轴进行照明和检测 电流计沿X轴扫描照明。这个第一阶段的项目 有五个具体目标。 我们将：1)设计并制造一台扫描场共聚焦显微镜；2)修改 现有数码相机采集软件制作证监会控制软件；3) 比较SFC和目前LSCM的光学效率和分辨率； 4)计算证监会的制造成本，并确定证监会是否 可能会以45,000美元的价格上市，最终测试一下 扫描场共聚焦显微镜在生物学中的应用。

DESCRIPTION (provided by applicant) Confocal microscopy is an essential tool for the biological sciences. It has significantly enhanced the testing of hypotheses in the Neurosciences and has provided insights into the causes of neurological disorders. The goal of this project is to develop a new type of confocal microscope based on a new concept, the swept-field confocal design (SFC). The SFC has the potential of becoming an attachment for the light microscope that could be purchased by the individual scientist for under $50,000. The SFC is a revolutionary confocal concept in which this hybrid-instrument uses an 86 x 12 array of stationary pinholes for illumination and detection along one axis (e.g. the Y axis) while using one galvanometer to sweep the illumination along the X axis. This phase I project has five specific objectives. We will: 1) Design and build a swept-field confocal microscope; 2) modify existing digital camera acquisition software to make SFC control software; 3) Compare the optical efficiency and resolution of the SFC and present day LSCMs; 4) Calculate the manufacturing costs of the SFC and determine whether the SFC could come to market at $45,000, and finally 5) Test the performance of the swept-field confocal microscope in biological applications.