CORE--NEUROPATHOLOGY

神经病理学核心提供完整的大脑银行和神经病理学 匹兹堡ADRC的诊断服务向银行保证 优质的组织与较短的死后时间，尸体解剖是 在24小时的基础上进行。大脑被矢状面一分为二 尸体解剖。右半部分被解剖以分配给ADRC 调查员和冻结的银行业务大脑的左半部分 固定并可用于磁共振成像。固定后 左半脑被广泛取样用于神经病理学诊断 包括H&E、刚果红、免疫细胞化学和银染色的测试。 左半脑的显微镜分析是根据 修订CERAD方案。除了有 神经退行性疾病的鉴别诊断， 发现具有阿尔茨海默病的变化是根据分期 公布了缠结密度的标准。大脑银行被分类， 与神经病理学诊断相关， 向ADRC和其他研究人员描述组织特征。神经病理 在一个月内向ADRC数据库和家庭提供诊断结果 死亡之截至1993年，神经病理学核心库大约有50个ADRC 每年的尸体解剖另外，将储存100个对照大脑， 以常规大体和显微镜神经病理学为特征 考试核心保持一个参考实验室（包括Ar/Kr 激光共聚焦显微镜和工作站）， 定量分析

The Neuropathology core provides complete brain banking and neuropathology diagnostic services for the Pittsburgh ADRC. To assure banking of excellent quality tissues with short post-mortem times, autopsies arc performed on a 24 hour basis. The brain is bisected sagittally at the time of autopsy. The right half is dissected for distribution to ADRC investigators and flash-frozen banking. The left half of the brain is fixed and made available for magnetic resonance imaging. After fixation the left hemi-brain is extensively sampled for neuropathology diagnostic tests including H&E, Congo Red, immunocytochemical and silver stains. Microscopic analysis of the left hemi-brain is performed according to a modification of the revised CERAD protocol. In addition to the differential diagnostics for neurodegenerative diseases, those brains found to possess changes of Alzheimer's Disease are staged according to published criteria of tangle densities. The brain bank is catalogued and correlated with neuropathology diagnoses to make available well characterized tissues to ADRC and other investigators. Neuropathology diagnoses are supplied to the ADRC database and families within one month of death. As of 1993 the neuropathology core banks approximately 50 ADRC autopsies per year. Additionally 100 control brains will be banked and characterized by routine gross and microscopic neuropathologic examination. The core maintains a reference laboratory (including a Ar/Kr laser confocal microscope and workstation) for development of detailed and quantitative analyses.