COMPARATIVE NEUROENDOCRINE CONTROL OF REPRODUCTION IN MOTHS

飞蛾繁殖的比较神经内分泌控制

• 批准号: 6204125
• 负责人: ASOKA S SRINIVASAN
• 金额: $ 6.64万
• 依托单位: TOUGALOO COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204125
• 关键词: Lepidoptera; behavioral /social science research tag; brain regulatory center; female; female reproductive system; fertility; gas chromatography mass spectrometry; genital secretion; gonadotropins; high performance liquid chromatography; hormone biosynthesis; hormone regulation /control mechanism; juvenile hormones; neuroendocrine system; oogenesis; reproduction; sex behavior; statistics /biometry; tissue /cell culture

During copulation, Heliothis virescens males transfer juvenile hormone (JH) to females and stimulate biosynthesis and release of her endogenous JH, while inhibiting JH degradation, thus stimulating egg maturation. This effect is hypothesized to be mediated via the brain, involving continued release of allatotropins that stimulates JH production by mated female corpora allata (CA) and simultaneously release of a JH-esterase inhibitor. Our ability to determine in vivo JH titers using gas chromatography-mass spectrometry and to measure in vitro JH biosynthesis and release by CA, combined with explicit physiological experiments show allow a determination of mechanisms involved in the mating-induced egg production seen in H. virescens. Heliothis virescens moths are readily available and easily reared in the laboratory. Numerous studies on the reproductive biology of this species suggest they are similar to other leptidopterans in many aspects of their reproductive physiology and, thus, serve as an excellent model to study these processes in Lepidoptera. The focus of this proposal is to study dynamics of JH titers in female Heliothis virescens, with the hypothesis that levels of endogenous JH decline with age in the virgin female and that on mating she derives a male factor that affects her brain, with a concomitant allatotropic and gonadotropic effect. The specific objectives of the proposed research to test this hypothesis are to: 1) determine the role of the female's brain- subesophageal ganglion (brain-SEG) complex in post-mating stimulation of egg production; 2) measure response of CA from mated and virgin female brain-SEG; and 4) sequence the H. virescens allatotropin. Results from experiments proposed, combined with data from concurrent studies on the nature of allatotropin/allatostatin in H. virescens, should provide a definitive explanation of the physiology of mating-induced gonadotropism in Lepidoptera, and also increase our knowledge of the interactions between neuropeptides and other endocrine secretions in the integrative physiology of animals. This project will be implemented in close collaboration with Dr. S.B. Ramaswamy, Chair and Professor, Department of Entomology, Kansas State University, who has also agreed to allow the investigator the use of his laboratory facilities.

在交配过程中，烟芽夜蛾雄性向雌性传递保幼激素（JH），刺激雌性内源性JH的合成和释放，同时抑制JH的降解，从而促进卵成熟。假设这种效应是通过大脑介导的，涉及持续释放的allatotropins，刺激JH生产交配雌性咽侧体（CA），同时释放的JH-酯酶抑制剂。我们的能力，以确定在体内JH滴度使用气相色谱-质谱和测量在体外JH的生物合成和释放CA，结合明确的生理实验表明，允许确定的交配诱导的鸡蛋生产中看到的H。绿色的烟实夜蛾是一种很容易获得和在实验室中很容易饲养的蛾。对该物种生殖生物学的大量研究表明，它们在生殖生理的许多方面与其他鳞翅目昆虫相似，因此，它们是研究鳞翅目昆虫生殖过程的一个很好的模型。这项建议的重点是研究动态的JH滴度在女性Heliothis virescens，与假设的内源性JH水平下降，随着年龄的处女女性和交配，她得出一个男性因素，影响她的大脑，伴随着allatotropic和促性腺激素的作用。为验证这一假设而提出的研究的具体目标是：1）确定雌性脑-食管下神经节（脑-SEG）复合体在交配后刺激产卵中的作用; 2）测量来自交配和处女雌性脑-SEG的CA反应; 4）对H.绿毛草素从实验结果提出，结合数据从并行研究的性质allatotropin/allatostatin在H。virescens，应该提供一个明确的解释交配诱导的促性腺激素的鳞翅目的生理学，也增加了我们的知识之间的相互作用的神经肽和其他内分泌物的综合生理学的动物。该项目将与S.B.博士密切合作实施。Ramaswamy，堪萨斯州立大学昆虫学系主任兼教授，他也同意让研究者使用他的实验室设施。