CORE--MOLECULAR BIOLOGY FACILITY

摘要：分枝杆菌感染的发病机制研究，包括 在过去的几年里，MAC得到了极大的帮助， 研究分枝杆菌分子遗传学的技术。此外，本发明还提供了一种方法， 分枝杆菌基因组测序计划已经提供了大量的 大量新的分子数据以及含有许多 在发病机制中的重要基因。这个核心的目的是使 这些预付款更容易提供给计划中的所有项目， 为i）DNA测序，ii）生长， 用于分枝杆菌的载体的制备、分类和储存 转化，iii）正常和突变体的生长、维持和储存 分枝杆菌菌株产生时; iv）生长维持和 储存含有目的基因的DNA克隆; v）生长、维持 和来自MAC菌株的DNA文库的储存。核心将采用 由一名技术人员执行这些功能并制造这些试剂 及时为所有项目提供服务。核心将采取 利用华盛顿大学现有的设施， 测序和序列分析。这一核心将有助于 通过提供专业知识，试剂， 以及分子生物学和遗传学技术所需的设施 所有项目的核心。此外，核心将作为一个重要的 通过保证分子质量、维持和分布， 所有项目都将使用的试剂和分枝杆菌突变体。

Abstract: The study of pathogenesis of Mycobacteria infections including MAC has been tremendously aided over the past few years by vastly improved techniques for studying molecular genetics of Mycobacteria. In addition, the mycobacteria genome sequencing projects have made available a large amount of new molecular data as well as cosmid clones containing many genes of importance in pathogenesis. The purpose of this core is to make these advances more readily available to all projects in the Program by providing consolidated facilities for i) DNA sequencing, ii) growth, preparation, cataloging, and storing of vectors for use in mycobacteria transformation, iii) growth, maintenance and storage of normal and mutant mycobacteria strains as they are generated; iv) growth maintenance and storage of DNA clones containing genes of interest; v) growth, maintenance and storage of DNA libraries from MAC strains. The core will employ a single technician to perform these functions and to make these reagents available to all projects in a timely fashion. The core will take advantage of facilities available at Washington University for automated sequencing and for sequence analysis. This core will be instrumental in achieving the objectives of the Program by providing expertise, reagents, and a facility required for the molecular biologic and genetic techniques central to all the projects. In addition, the core will serve an essential role by assuring the quality, maintenance, and distribution of molecular reagents and mycobacteria mutants which will be used by all projects.