CORE--MOLECULAR BIOLOGY FACILITY

摘要：分枝杆菌感染的发病机制研究包括 在过去的几年里，Mac得到了极大的改善，得到了极大的帮助 分枝杆菌分子遗传学研究技术。此外, 分枝杆菌基因组测序项目已经提供了大量的 大量新的分子数据以及包含许多 发病机制中的重要基因。这个核心的目的是使 通过以下方式，计划中的所有项目都可以更容易地获得这些进步 为i)DNA测序、ii)生长、 用于分枝杆菌的载体的准备、编目和存储 转化，III)正常和突变的生长、维持和储存 分枝杆菌菌株的产生；iv)保持生长和 含有目的基因的DNA克隆的保存；v)生长、维持 以及MAC菌株DNA文库的保存。核心将采用一种 一名技术人员执行这些功能并制造这些试剂 及时提供给所有项目。它的核心将需要 华盛顿大学提供的自动化设施的优势 测序和序列分析。这一核心将有助于 通过提供专业知识、试剂、 以及分子生物学和遗传技术所需的设施 是所有项目的核心。此外，核心将服务于一个必不可少的 通过确保分子的质量、维护和分布来发挥作用 所有项目都将使用的试剂和分枝杆菌突变体。

Abstract: The study of pathogenesis of Mycobacteria infections including MAC has been tremendously aided over the past few years by vastly improved techniques for studying molecular genetics of Mycobacteria. In addition, the mycobacteria genome sequencing projects have made available a large amount of new molecular data as well as cosmid clones containing many genes of importance in pathogenesis. The purpose of this core is to make these advances more readily available to all projects in the Program by providing consolidated facilities for i) DNA sequencing, ii) growth, preparation, cataloging, and storing of vectors for use in mycobacteria transformation, iii) growth, maintenance and storage of normal and mutant mycobacteria strains as they are generated; iv) growth maintenance and storage of DNA clones containing genes of interest; v) growth, maintenance and storage of DNA libraries from MAC strains. The core will employ a single technician to perform these functions and to make these reagents available to all projects in a timely fashion. The core will take advantage of facilities available at Washington University for automated sequencing and for sequence analysis. This core will be instrumental in achieving the objectives of the Program by providing expertise, reagents, and a facility required for the molecular biologic and genetic techniques central to all the projects. In addition, the core will serve an essential role by assuring the quality, maintenance, and distribution of molecular reagents and mycobacteria mutants which will be used by all projects.