CORE--MEDIA PREPARATION

UAB综合癌症中心（UAB- ccc）的培养基制备共享设施（MPSF）是以下方面的集中资源：(a)真核和细菌细胞培养基和补充剂，(b)常规细胞培养和分子生物学应用中使用的缓冲液和其他大容量试剂，(c)质量控制保证和测试，(d)关于细胞培养和分子生物学试剂的一般咨询和教育。总体目标是提高UAB-CCC研究活动的经济性、效率和质量。MPSF通过向UAB-CCC教师提供普通培养基、补充物和缓冲液，以低于现有商业价格的回收费提供经济效益。这是通过以下方式实现的：(1)现场生产，退款率不能产生额外的MPSF收入；(2)与商业供应商进行批量采购谈判，从而降低价格和运费，这是一般研究实验室无法实现的。MPSF通过方便的现场产品/服务提供效率，与商业供应商相比，文书工作更少，交付速度更快。许多用户在最初请求后不到2小时就拥有了MPSF产品。MPSF通过(a)在现场方案中定期进行内毒素和无菌检测，以及在研究者提交的样品中进行内毒素和支原体检测，以及现场高压灭菌器性能来提高质量，以及(b)提供个性化服务，包括针对特定实验问题的免费专业咨询，以及以低成本设计和制备定制试剂。

The Media Preparation Shared Facility (MPSF) of the UAB Comprehensive Cancer Center (UAB-CCC) serves as a centralized resource for (a) eukaryotic and bacterial cell culture media and supplements, (b) buffers and other high-volume reagents used in routine cell culture and molecular biology applications, (c) quality control assurance and testing, and (d) general consultation and education with regard to cell culture and molecular biology reagents. The overall goals are to improve economy, efficiency and quality of UAB-CCC research activities. MPSF provides economy by supplying common culture media, supplements and buffers to UAB-CCC faculty at charge-back prices lower than available commercial prices. This is accomplished via (1) on- site production with charge-back rates that fail to generate excess MPSF income, and (2) volume purchase negotiations with commercial suppliers yielding price and shipping charge reductions not available to the average research laboratory. MPSF offers efficiency by convenient on-site product/service availability, with less paperwork and faster delivery as compared to commercial suppliers. Many users take possession of MPSF products in less than 2 hours after initial request. MPSF promotes quality by (a) regularly performing assays for endotoxin and sterility in on-site protocols, as well as for endotoxin and mycoplasma in investigator-submitted samples, and on-site autoclave performance, and (b) offering personalized services including professional consultation for specific experimental problems at no cost, plus design and preparation of custom reagents at low cost.