CORE--MEDIA PREPARATION

描述：（申请人的描述）培养基制备共享设施 UAB综合癌症中心（UAB-CCC）的MPSF（MPSF）是一个 用于（a）真核和细菌细胞培养基的集中资源，和 补充剂，（B）缓冲液和其他常规细胞培养中使用的高容量试剂 培养和分子生物学应用，（c）质量控制保证， 测试，以及（d）关于细胞的一般咨询和教育 培养和分子生物学试剂。总体目标是提高 UAB-CCC研究活动的经济性、效率和质量。 MPSF通过提供普通培养基、补充剂和 以低于现有价格的收费价格向UAB-CCC教师提供缓冲 商业价格。这是通过（1）现场生产， 无法产生超额MPSF收入的退款率，以及（2）数量 与商业供应商进行采购谈判，确定价格和运输 这是一般研究实验室所无法获得的收费减免。 MPSF通过方便的现场产品/服务可用性提供效率， 与商业供应商相比，具有更少的文书工作和更快的交付。 许多用户在最初购买MPSF产品后不到2小时即可获得产品 请求过程中 MPSF通过（a）定期进行内毒素测定， 现场产品的无菌性，以及 灭菌器提交的样品和现场灭菌器性能，以及（B） 提供个性化服务，包括专业咨询， 具体的实验问题，在没有成本，加上设计和准备， 低成本定制试剂。

Description:(Applicant's Description) The Media Preparation Shared Facility (MPSF) of the UAB Comprehensive Cancer Center (UAB-CCC) serves as a centralized resource for (a) eukaryotic and bacterial cell culture media and supplements, (b) buffers and other high-volume reagents used in routine cell culture and molecular biology applications, (c) quality control assurance and testing, and (d) general consultation and education with regard to cell culture and molecular biology reagents. The overall goals are to improve economy, efficiency and quality of UAB-CCC research activities. MPSF provides economy by supplying common culture media, supplements and buffers to UAB-CCC faculty at charge-back prices lower than available commercial prices. This is accomplished via (1) on-site production with charge-back rates that fail to generate excess MPSF income, and (2) volume purchase negotiations with commercial suppliers, yielding price and shipping charge reductions not available to the average research laboratory. MPSF offers efficiency by convenient on-site product/service availability, with less paperwork and faster delivery as compared to commercial suppliers. Many users take possession of MPSF products in less than 2 hours after initial request. MPSF promotes quality by (a) regularly performing assays for endotoxin and sterility in on-site products, as well as for endotoxin and mycoplasma in investigator-submitted samples, and on-site autoclave performance, and (b) offering personalized services including professional consultation for specific experimental problems at no cost, plus design and preparation of custom reagents at low cost.