SENSORY PROCESSING BY SUBFORNICAL ORGAN IN BODY FLUID/CARDIOVASCULAR HOMEOSTASIS

体液和心血管的动态平衡取决于摄食行为 以及作用于调节肾脏水分和钠流失的反射 重新分配血液流动。为了使适当的效应器机构 维持体液平衡和心血管功能 参与时，大脑必须接收有关当前状态的信息 身体的水分和血压。几个内脏感官系统 向中枢神经系统(CNS)提供这种类型的信息。这 输入通过传入神经通路传递，并由 选定的缺乏血液脑的脑区的循环因子 障碍。大脑的三个可渗透区域接收血液传播的信号 被描述为感觉周脑室器官(CVO)，并 以穹隆下器(SFO)为代表。 这项建议集中在SFO及其在检测循环中的作用 血管紧张素II，这种信息是如何在这个结构中处理的，以及 最终转移到参与行为的前脑区域 以及对水矿物质平衡和血压的反射控制。现在 建议建立在我们之前对中枢感觉和 体液和心血管传入信号的处理 动态平衡。本研究的目的是探讨其作用机制。 感觉传递和神经处理与SFO的反应 通过血液传播的血管紧张素Ⅱ，然后描述传递这一信息的途径 到参与维持的特定前脑核团的信息 心血管和体液动态平衡。这项研究将利用这两个项目 电生理学、药理学和生物活性的体内和体外方法 用SFO对已鉴定的细胞进行解剖学鉴定。牢房将会是 分析以了解他们在内部和内部使用的机制 细胞信号。从对这一重要问题的研究中获得的新信息 将体循环与中枢神经系统相结合的感觉结构 恰当地理解正常生理学和 与体液紊乱状态相关的病理生理学 心血管调节，如在高血压中。

Body fluid and cardiovascular homeostasis depends on ingestive behaviors and on reflexes acting to modulate renal water and sodium loss and to redistribute blood flow. In order for the appropriate effector mechanisms that maintain body fluid balance and cardiovascular function to be engaged, the brain must receive information about the current status of body hydration and blood pressure. Several visceral sensory systems provide this type of information to the central nervous system (CNS). This input is carried over afferent neural pathways and by the direct action of circulating factors on select brain regions which lack a blood-brain barrier. Three permeable areas of the brain receiving blood-borne signals have been described as sensory circumventricular organs (CVOs) and are typified by the subfornical organ (SFO). This proposal focuses on the SFO and its role in detecting circulating angiotensin II, how this information is handled within this structure and ultimately transferred into forebrain regions involved in the behavioral and reflex control of hydromineral balance and blood pressure. The present proposal builds upon our prior studies of the central sensing and processing of afferent signals involved in body fluid and cardiovascular homeostasis. The aims of this research are to investigate the mechanisms of sensory transfer and neural processing with the SFO in response to blood-borne ANG II and then to characterize the pathways which convey this information to specific forebrain nuclei involved in maintaining cardiovascular and body fluid homeostasis. The studies will employ both in vivo and in vitro methods to electrophysiologically, pharmacologically and anatomically characterize identified cells with the SFO. The cells will be analyzed to understand the mechanisms they use for both intra- and inter- cellular signaling. New information derived from studies on this important sensory structure that couples the systemic circulation with the CNS proper has relevance for understanding both normal physiology and pathophysiology associated with states of disordered body fluid and cardiovascular regulation, such as in hypertension.