FISH ASSAY FOR THE DETECTION OF T (14;18) (Q32;21)

用于检测 T (14;18) (Q32;21) 的鱼测定

• 批准号: 6294011
• 负责人: Nallasivam Palanisamy
• 金额: $ 41.8万
• 依托单位: CANCER GENETICS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-10 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6294011
• 关键词: chromosome translocation; fluorescent in situ hybridization; human genetic material tag; human subject; minimal residual disease; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplastic cell; nonHodgkin's lymphoma; nucleic acid probes; technology /technique development

DESCRIPTION (applicant's abstract): Approximately 60 percent of B-cell lymphomas are characterized by the presence of the t(14;18) (q32;q21) chromosomal translocation. Diagnosis and post-treatment follow-up are currently performed by karyotype, Southern blotting, or PCR, which have problems with specificity and sensitivity. Fluorescence in situ hybridization (FISH) addresses these limitations; however, no clinically tested FISH probes are available for detecting the t(14; 18) translocation. Phase I research developed novel probes for interphase FISH analysis of the t(14; 18) translocation. Using both normal and tumor samples, high sensitivity and specificity of the probes were observed. Using tumor specimens from multiple tissue types, Phase II research will validate use of the probes for diagnosis. Phase II research will also validate their use for monitoring minimal residual disease. In Phase II research, the probes will also be optimized to reduce background hybridization signal. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（申请人摘要）：约60%的b细胞