LIPOPROTEIN-PROTEOGLYCAN INTERACTIONS IN DIABETES

该项目旨在研究糖尿病对糖尿病患者的潜在作用。 脂蛋白和细胞外蛋白多糖之间的相互作用 动脉壁，其改变可能导致 动脉粥样硬化的风险典型的糖尿病。 将进行研究， 确定是否脂蛋白或蛋白聚糖的改变， 可能发生在糖尿病患者身上，会以这种方式改变它们的相互作用， 会导致动脉壁中脂蛋白的滞留增加。 胰岛素依赖型糖尿病和非胰岛素依赖型糖尿病的共同特征，如脂蛋白氧化，非胰岛素依赖型糖尿病， 蛋白质的酶促糖化，改变基质基因表达，以及 假设肾脏并发症的发生会改变脂蛋白- 蛋白聚糖相互作用 这些相互作用也可能受到 载脂蛋白E和脂蛋白等中间分子的存在和性质 脂肪酶（LPL）。 脂蛋白-蛋白聚糖相互作用将通过以下方法进行评价： 通过研究糖尿病患者体内的脂蛋白， 和肾脏并发症，以及来自受试者和肾脏的蛋白多糖 并发症和来自糖尿病受试者动脉的蛋白聚糖。 项目2是一个综合方案的一部分， 了解加速性大血管疾病的机制， 糖尿病 因此，它与其他四个项目相互作用， 这个计划项目。

This project proposes to investigate the potential role of diabetes on the interaction between lipoproteins and extracellular proteoglycans of the artery wall, alterations of which might account for the increased atherosclerosis risk typical of diabetes. Studies will be undertaken to determine whether alterations in either lipoproteins or proteoglycans that might occur in diabetes will modify their interaction in such a manner that would result in the increased retention of lipoproteins in the artery wall. Features common to IDDM and NIDDM such as lipoprotein oxidation, non- enzymatic glycation of proteins, altered matrix gene expression, and development of renal complications are hypothesized to alter lipoprotein- proteoglycan interaction. These interactions may also be affected by the presence and nature of intermediary molecules such as apo E and lipoprotein lipase (LPL). Lipoprotein-proteoglycan interactions will be evaluated by in vitro experiments, by studying lipoproteins from subjects with diabetes and renal complications, and proteoglycan from subjects and renal complications, and proteoglycans from arteries of subjects with diabetes. Project 2 is part of an integrated program aimed at providing further understanding of the mechanisms of accelerated macrovascular disease in diabetes. As such, it interacts with each of the four other projects in this Program Project.