CELL AND TISSUE CORE

说明（申请人提供） 细胞和组织核心（CTC）通过整合由于需要特殊培训和设备而不易获得的几项专业活动，鼓励和支持附属研究者使用组织培养和分离组织。这样做的一个实际结果是，这些高度专业化的服务以统一的高产量和高质量提供。反恐委员会为33名调查员提供了支助，其项目见核心部分的科学基础。这项研究涵盖了广泛的糖尿病相关主题，从分子生物学到1型糖尿病和2型糖尿病以及糖尿病并发症的大型流行病学研究，包括：1型糖尿病的免疫学、胰岛细胞生物学和生理学、燃料稳态和肥胖、糖尿病血脂异常和动脉壁细胞的细胞生物学，以病人为中心的医学研究，调查糖尿病及其并发症的危险因素。由于气候技术中心，许多这些项目的主要部分得以实施。CTC提供1）组织培养相关设备; 2）培养基、平衡盐溶液和缓冲液; 3）来自中央储存库的细胞和细胞培养物; 4)血沉棕黄层和DNA; 5）细胞转化和转染; 6）器官分离和培养; 7）实验技术援助; 8）杂交瘤技术援助; 9）组织培养方法学方面的工作人员和研究员培训; 10）咨询。核心管理和设施、服务、开发工作、培训活动和福利在核心职能下描述。使用核心文献的研究者发表的文献列于核心文献目录中。

Description (Provided by applicant) The Cell and Tissue Core (CTC) stimulates and supports the use of tissue culture and isolated tissues by Affiliate Investigators by consolidating several specialized activities that would not otherwise be easily accessible due to a need for special training and equipment. A practical outcome of this is that these highly specialized services are provided at uniform high yield and quality. The CTC supported 33 investigators whose projects are described in the Scientific Base of the Core section. This research covers a broad range of diabetes-related topics, from molecular biology to large, epidemiologic studies of type 1 diabetes and type 2 diabetes and diabetic complications, including: the immunology of type 1 diabetes, pancreatic islet cell biology and physiology, fuel homeostasis and obesity, diabetic dyslipidemia and cell biology of arterial wall cells, and patient-oriented medical research investigating risk factors for diabetes and its complications. Major portions of many of these projects were made possible because of the CTC. The CTC provides 1) access to tissue culture-related equipment; 2) culture medium, balanced salt solutions, and buffers; 3) cells and cell cultures from a central repository; 4) buffy coats and DNA; 5) cell transformations and transfections; 6) organ isolations and cultures; 7) technical assistance with experiments; 8) technical assistance with hybridomas; 9) training of staff and fellows in tissue culture methodology; and 10) consultation. Core administration and facilities, services, development work, training activities, and benefits are described under Core Function. Publications by investigators who have used the Core are listed in the Core Bibliography.