Retrieval of DNA, RNA and Protein from Archival Tissues

描述：（申请人提供） 虽然普遍使用和信息，以获得免疫组织化学 病理资料是宝贵的临床档案资源 福尔马林固定的石蜡包埋组织由于受到限制而未得到充分利用。 能够有效地回收蛋白质、DNA和RNA。改善 因此，使这些生物分子的提取最大化的技术， 这对癌症研究领域至关重要， 结合经典病理学和现代分子生物学技术， 完成回顾性分子形态学研究。建议的研究 本申请的目的在于实现对 所有这三种大分子都来自储存的固定组织。哲学 检索有其基础，在详尽的研究工作，开创了这一点。 实验室在提供否则无法获得的生物分子， 对存档固定的组织切片进行免疫组织化学染色。基本 福尔马林与蛋白质和核酸的化学反应包括 肽和核苷酸中的氨基或亚氨基被 羟甲基然后进行二次缩合反应， 蛋白质和核酸，导致形成稳定的 亚甲基化合物

DESCRIPTION: (provided by applicant) Although used universally and informatively to obtain immunohistochemical pathology data, the invaluable clinical resource in the form of archival formalin-fixed, paraffin-embedded tissues is under-utilized due to restricted ability to effectively retrieve proteins, DNA and RNA. Improvements in the techniques to maximize extraction of these bio-molecules are, therefore, critical to the field of cancer research, which would enable effective combination of classical pathology and modern molecular biology techniques to accomplish retrospective molecular morphology studies. The studies proposed in this application are aimed at achieving substantial improvements in yield of all these three macromolecules from stored fixed tissues. The philosophy of retrieval has its foundation in the exhaustive research work pioneered by this laboratory in rendering otherwise inaccessible bio-molecules available for immunohistochemical staining on archival fixed tissue sections. The basic chemical reactions of formalin with protein and nucleic acid include a replacement of amino or imino groups in peptides and nucleotides by hydroxymethyl group. This is followed by secondary condensation reaction for both proteins and nucleic acids, resulting in the formation of a stable methylene compound.