Retrieval of DNA, RNA and Protein from Archival Tissues

描述：(申请人提供) 虽然普遍和信息用于获得免疫组化 病理资料--档案形式的宝贵临床资源 福尔马林固定的石蜡包埋组织由于受到限制而未得到充分利用 能够有效地提取蛋白质、DNA和RNA。改进了 因此，最大化提取这些生物分子的技术是， 对癌症研究领域至关重要，这将使有效的 经典病理学和现代分子生物学技术相结合 完成回溯性分子形态研究。建议进行的研究 这项应用的目的是实现产量的大幅提高 所有这三个大分子都是从储存的固定组织中提取的。人生观 检索的基础是由它开创的详尽的研究工作 实验室呈现原本无法获得的生物分子 档案固定组织切片的免疫组织化学染色。最基本的 福尔马林与蛋白质和核酸的化学反应包括 取代多肽和核苷酸中的氨基或亚氨基 羟甲基。然后是二次缩合反应，用于 蛋白质和核酸，导致形成稳定的 亚甲基化合物。

DESCRIPTION: (provided by applicant) Although used universally and informatively to obtain immunohistochemical pathology data, the invaluable clinical resource in the form of archival formalin-fixed, paraffin-embedded tissues is under-utilized due to restricted ability to effectively retrieve proteins, DNA and RNA. Improvements in the techniques to maximize extraction of these bio-molecules are, therefore, critical to the field of cancer research, which would enable effective combination of classical pathology and modern molecular biology techniques to accomplish retrospective molecular morphology studies. The studies proposed in this application are aimed at achieving substantial improvements in yield of all these three macromolecules from stored fixed tissues. The philosophy of retrieval has its foundation in the exhaustive research work pioneered by this laboratory in rendering otherwise inaccessible bio-molecules available for immunohistochemical staining on archival fixed tissue sections. The basic chemical reactions of formalin with protein and nucleic acid include a replacement of amino or imino groups in peptides and nucleotides by hydroxymethyl group. This is followed by secondary condensation reaction for both proteins and nucleic acids, resulting in the formation of a stable methylene compound.