COMPLETE GENOME SEQUENCE OF RICKETTSIA TYPHI

伤寒立克次体全基因组序列

• 批准号: 6299108
• 负责人: DAVID H WALKER
• 金额: $ 30.77万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299108
• 关键词: DNA; Rickettsia; bacteria infection mechanism; bacterial genetics; bioterrorism /chemical warfare; genetic mapping; genetic techniques; genome; molecular biology information system; nucleic acid sequence; virulence

The complete DNA sequences of the genome of Rickettsia typhi will be determined and analyzed for putative coding regions and possible functions of the gene products. Under an addendum for potential bioterrorism agents of the Policy on Support of Large- Scale Genome Sequencing Projects, R. typhi has been designated as a priority bioterrorism agent. This application is prepared in response to this opportunity for support of large-scale genome sequencing of high priority pathogens as potential agents of bioterrorism. Rickettsia typhi is highly infectious, including via aerosol exposure, infection is deceptively difficult to diagnose clinically and causes a severe, incapacitating illness with 1 percent overall mortality. Knowledge of its genome would be most useful for comparison with that of the more virulent R. prowazekii and for investigation of virulence factors, targets of attenutation, and potential novel targets for antimicrobial therapy of strains that could be engineered to resistance to the only two effective agents, tetracyclines and chloramphenicol. Once the genomic sequences are determined and analyzed they will be made available to the research community through an online database with a number of tools to facilitate data retrieval. During the course of the project there will also be ongoing release of preliminary data.

将测定伤寒立克次体基因组的完整DNA序列，并分析推定的编码区和基因产物的可能功能。 根据《支持大规模基因组测序项目政策》关于潜在生物恐怖主义制剂的附录，R。伤寒已被指定为生物恐怖主义的优先制剂。 本申请是为了响应这一机会而编写的，以支持作为生物恐怖主义潜在制剂的高优先级病原体的大规模基因组测序。 伤寒立克次体具有高度传染性，包括通过气溶胶暴露，感染在临床上很难诊断，并导致严重的失能性疾病，总死亡率为1%。 它的基因组知识将是最有用的比较与更致命的R。prowazekii和研究毒力因子、减毒靶点和用于菌株的抗微生物治疗的潜在新靶点，所述菌株可以被工程化以抵抗仅有的两种有效药剂四环素类和氯霉素。一旦基因组序列被确定和分析，它们将通过一个在线数据库提供给研究界，该数据库具有许多工具，以方便数据检索。在项目实施过程中，还将不断发布初步数据。