EXPERIMENTAL INFECTION OF THE MARMOSET WITH LASSA VIRUS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拉萨(LAS)病毒是一种东半球的阿拉伯病毒，也是拉萨出血热(LHF)的病原体，每年在西非造成多达30万人感染。大约30%的感染导致各种疾病，从轻微的流感样疾病到致命的LHF，每年导致数千人死亡。巨大的疾病负担、严重的并发症、致命性以及LAS病毒可用作生物战剂的可能性，为有效开发疫苗提供了强有力的理由。恒河猴传统上被选为研究病毒性出血热(VHF)病毒的模型，但不幸的是，容纳这些动物所需的BSL-4空间有限。随着针对VHF病毒的生物防御产品资金的增加，对BSL-4空间进行这些研究的需求将会增加。目前的研究将小体绒猴作为LHF的替代模型进行研究。在最大限度的控制下，绒猴将被实验性地感染Lassa Josiah。在感染过程中，将采集血液进行血细胞分析、病毒载量测定和蛋白质组学分析。在项目结束时，将收集组织进行组织学检查。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lassa (LAS) virus, an Old World Arenavirus and the etiological agent of Lassa hemorrhagic fever (LHF), causes up to 300,000 annual infections in West Africa. Approximately 30% of infections result in disease varying from mild influenza-like illness to lethal LHF causing several thousand deaths per year. The large disease burden, severe complications, lethality and the possibility that LAS virus can be used as a biological warfare agent make a strong case for effective vaccine development. Rhesus macaques have traditionally been chosen as models to study viral hemorrhagic fever (VHF) viruses, unfortunately, the amount of BSL-4 space required to house these animals is limited. With the increase in funding for biodefense products against VHF viruses, the demand for BSL-4 space to perform these studies will increase. The current study investigates the small-bodied marmoset as an alternative model for LHF. Under maximum containment, marmosets will be experimentally infected with Lassa Josiah. During the course of infection blood will be collected for CBC analysis, viral load determination and proteomic profiling. At project end tissues will be collected for histological examination.