CORE--LIGAND PREPARATION AND ASSAY

配基分析和分析核心将服务于几乎所有符合条件的 此U54中心应用程序中确定的项目。简而言之，核心 将：(1)制备和纯化用于分析的标记配体， 激动剂/拮抗剂结合研究或免疫细胞化学；(2)提供 对激素或其他调节分子浓度的估计 通过放射免疫分析、化学发光、免疫测定和生物测定程序；(3) 将相关的配基制备和检测技术程序转移到 项目；以及(4)开发(10%的努力)所需的新的或改进的程序 根据符合核心资助条件的资助项目不断变化的调查需求 进入。 建立这个核心实验室的主要动机是提供 为U54中心相关项目提供高质量的技术服务 需要分析试剂、标记的配体和荷尔蒙的评估 浓度。这项服务将使多个U54和生殖系统受益 科学项目，因为没有其他机制可以获得 所需的大容量分析和/或高纯度配体 用分离的细胞或颗粒碎片进行研究 生殖细胞或组织。需要新配体或化验的项目 不能证明技术人员花费时间或精力来建立、验证 并执行这些程序。中央设施将允许U54中心 满足所有项目的需求，提高其生产力，实施 提出这个核心的一套统一的理论基础是提供一个有效的 新的或改进的(包括非放射性)方法可以 用于估计多肽、生长因子和 多种细胞培养和体液中的类固醇。本配基分析和 因此，分析核心将服务于符合条件的客户的当前和未来需求 这个U54中心的生殖科学项目需要专门的 配基和化验中心设施，用于进行、开发、验证和 实施相关的测量和分析技术。

The Ligand Assay and Analysis Core will serve virtually all eligible projects identified in this U54 Center application. In brief, the Core will: (1) prepare and purify labeled ligands for assays, agonist/antagonist binding studies, or immunocytochemistry; (2) provide estimates of the concentration of hormones or other regulatory molecules by RIA, chemiluminescent, immunometric, and bioassay procedures; (3) transfer relevant ligand preparation and assay technology procedures to projects; and (4) develop (10% effort) new or improved procedures required by the evolving investigative needs of funded projects eligible for Core access. The primary drive for establishing this Core laboratory is to provide high-quality technical services to U54 Center-related projects that require assay reagents, labeled ligands, and estimates of hormone concentrations. This services will benefit multiple U54 and Reproductive Sciences projects, since no mechanisms are available otherwise to obtain high-volume assays and/or high purified ligands required for investigations with isolated cells or particulate fractions of reproductive cells or tissue. Projects requiring new ligands or assays cannot justify the time or effort of a technician to establish, validate, and perform these procedures. A central facility will allow the U54 Center to meet the needs of all projects, enhance their productivity, implement a uniform set rationale for proposing this Core is to provide an efficient mechanism whereby new or improved (including non-radioactive) methods can be implemented for estimating amounts of peptides, growth factors, and steroids in multiple cell-culture and body fluids. This Ligand Assay and Analysis Core will thus serve the immediate and future needs of eligible reproductive-science projects in this U54 Center that require a dedicated ligand and assay central facility to conduct, develop, validate, and implement relevant measurement and analysis techniques.