FLUORESCENCE STUDIES OF BIOPHYSICAL PROPERTIES OF POLYUN

Polyun生物物理性质的荧光研究

• 批准号: 6431359
• 负责人: Burton J Litman
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6431359
• 关键词: calorimetry; chemical models; ethanol; fluorescence; fluorescent dye /probe; lipid bilayer membrane; long chain fatty acid; membrane activity; membrane lipids; membrane structure; phase change; phosphatidylcholines; phospholipids; retina; synaptosomes; unsaturated fatty acids

Neuronal and retinal tissues are high in phospholipids containing one or two long chain polyunsaturated acyl chains. These studies are directed towards determining a molecular basis for the modulation of G protein-coupled receptor signaling by phospholipids containing polyunsaturated acyl chains, in particular 22:6n-3. Fluorescence Energy Transfer (FRET) was used to determine the degree of lateral domain formation in bilayers containing di16:0 and di22:6 PCs plus cholesterol. Lateral domain formation, which was dependent on the presence of rhodopsin, was detected. Polyunsaturated acyl chains result in the highest level of formation of the active conformation of the G protein-coupled receptor, rhodopsin. The role of lateral domain formation in bilayers containing 22:6n-3 containing lipids is being further investigated. Other experiments indicate that ethanol may disrupt the lateral domains, suggesting a potential mechanism by which ethanol can modulate membrane function. Both acyl chain free volume and curvature stress have been invoked as bilayer properties, which modulate membrane protein function. Experiments are currently being carried out to determine the relationship between these two properties. These studies are important in understanding the role of 22:6n-3 acyl chains in domain formation, the mechanism of alcohol action, and the modulation of membrane protein function

神经元和视网膜组织中含有大量含有一个或两个长链多不饱和酰基链的磷脂。 这些研究是针对确定由含有多不饱和酰基链，特别是22：6 n-3的磷脂调节G蛋白偶联受体信号传导的分子基础。 荧光能量转移（FRET）被用来确定在双层含有二16：0和二22：6 PC加胆固醇的横向域形成的程度。横向结构域的形成，这是依赖于视紫红质的存在下，被检测到。 多不饱和酰基链导致G蛋白偶联受体视紫红质的活性构象形成的最高水平。 在含有22：6 n-3的脂质的双层中，侧域形成的作用正在进一步研究中。 其他实验表明，乙醇可能会破坏侧域，这表明乙醇可以调节膜功能的潜在机制。 酰基链自由体积和曲率应力都被认为是调节膜蛋白功能的双层性质。 目前正在进行实验，以确定这两个属性之间的关系。 这些研究对于理解22：6 n-3酰基链在结构域形成中的作用、酒精作用机制以及膜蛋白功能的调节具有重要意义