EFFICACY STUDIES OF CHEMOPREVENTIVE AGENTS IN ANIMAL MOD

使用雌性Lewis大鼠研究各种化学预防药物减少或抑制卵巢癌的效果。雌性Lewis大鼠正在接受S.C.注射。10日龄和14日龄分别给予0.8 mg N-亚硝基-(2-氧丙基)胺(BOP)/kgBW。21天断奶后，雌性大鼠(每组30只)在研究期间开始在饲料中注射或灌胃CP制剂。这项研究持续了大约30周(直到老鼠大约8个月大)。要给药的剂量是在六周的测距试验后确定的。每种药剂要服用两剂。每周给这些动物称重并触诊卵巢肿块一次。在研究结束时(约8个月)摘除卵巢，并进行组织学检查。记录肿瘤的发生率、多发性、组织学分类和部位。被测试的试剂是：甲羟孕酮(20日龄注射，然后每两个月注射一次)，抗雌激素(TBN)，甲羟孕酮+抗雌激素，9-顺式维甲酸，4HPR和阿波明(SR-45023A)(Symphar，Inc.)。所有小组都进行了编码，小组由两个独立的评分员以盲法评分。对所有数据进行适当的统计分析，将确定各种药剂增强或抑制效果的重要性。正在研究的药物类别包括维甲酸、抗雌激素和卵巢特异药阿波明。

The efficacy of a variety of chemopreventive agents to reduce or inhibit ovarian cancer using female Lewis rats is being studied. Female Lewis rats are being injected s.c. with 0.8 mg N-nitroso-(2-oxopropyl)amine (BOP) /kgBW at 10 and 14 days of age. After the 21 day weaning period the female (30/group) rats begin receiving CP agents either in the diet, injected or by gavage for the duration of the study. The study runs approximately 30 weeks (until the rats are ~8 months old). The doses to be administered are determined following a six week rangefinding assay. Two doses per agent are administered. The animals are weighed and palpated for ovarian masses once per week. The ovaries are removed at the end of the study (~8 months) and histologically examined for lesions. The incidence, the multiplicity, the histological classification and locations of the tumors are recorded. The test agents to be tested are: Medroxyprogesterone (injected at 20 days of age then bimonthly), an antiestrogen (TBN), Medroxyprogesterone+Antiestrogen, 9-cis-retinoic acid, 4HPR, and Apomine (SR-45023A) (Symphar, Inc). All groups are coded and groups are scored in a blinded manner by two independent scorers. An appropriate statistical analysis on all data will determine significance of enhancing or inhibiting effects of the various agents. The classes of agent being studied include retinoids, antiestrogenic, and an ovarian specific drug Apomine.