ISOLATION & CHARACTERIZATION OF PRIMATE BIOMACROMOLECULES

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• 批准号: 6316824
• 负责人: JOHN R CASHMAN
• 金额: $ 5.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316824
• 关键词: animal tissue; biotechnology; drug adverse effect; enzymes; human tissue; inborn metabolism disorder; liver; nervous system; structural biology

One liver sample from each animal was obtained under sterile conditions. Total RNA was extracted from the liver sample and purified by standard procedures. RNA obtained was converted into DNA by the action of the enzyme reverse transcriptase using standard protocols. Currently, selective oligonucleotide probes are being used to identify specific genes in the transcribed DNA. FUNDING NIH grant RR00166

在无菌条件下从每只动物获得一份肝脏样品。 条件 从肝脏样品中提取总RNA， 通过标准程序纯化。 将获得的RNA转化为DNA 通过逆转录酶的作用， 协议. 目前，正在使用选择性寡核苷酸探针 来识别转录DNA中的特定基因。 资助NIH赠款 RR00166