MORPHOLOGICAL ANALYSIS OF COLON TISSUES USING UV RESONANCE RAMAN

使用紫外共振拉曼对结肠组织进行形态分析

• 批准号: 6314189
• 负责人: NADA BOUSTANY
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314189
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; biomedical resource; human tissue; microscopy; model design /development; technology /technique

The objective of this study is understand the morphological and biochemical origin of resonance Raman signals from normal, precancerous and cancerous colon tissues. When used in reverse, we will be able to extract quantitative morphological information from tissue and predict the patholgy. So far we have studied normal and pathological samples from six patients using 251 nm UV excitation light. MicroRaman spectrometer was used to collect spectra of various mophological structures including normal cells, precancer cells, carcinoma cells, collagen fibers in lamina propria and submucosa, and adipose tissue. 251 nm excited UV resonance Raman spectra of bulk normal, adenomatous and adenocarcinoma cells have been modeled in terms of the above morphological structures. The results will be compared with those of standard morphometric analysis.

这项研究的目的是了解植物的形态和 正常人共振拉曼信号的生化来源， 癌前病变和癌变的结肠组织。当反转使用时，我们 将能够提取定量的形态信息 组织和预测病理。到目前为止，我们已经研究了正常和 用251 nm紫外光激发6例患者的病理标本 灯。用显微拉曼光谱仪采集了不同样品的光谱。 形态结构包括正常细胞、癌前细胞、 癌细胞，固有层和粘膜下层的胶原纤维，以及 脂肪组织。块体的251 nm激发紫外共振拉曼光谱 正常细胞、腺瘤细胞和腺癌细胞已经在 上述形态结构的术语。结果将是 与标准形态计量学分析结果进行了比较。