MORPHOLOGICAL ANALYSIS OF COLON TISSUES USING UV RESONANCE RAMAN

本研究的目的是了解形态学和 共振拉曼信号的生化来源， 癌前和癌性结肠组织。 当反向使用时，我们 将能够提取定量的形态学信息， 组织并预测病理。 到目前为止，我们已经研究了正常和 使用251 nm UV激发从六名患者的病理学样品 光 显微拉曼光谱仪用于收集各种 包括正常细胞，癌前细胞， 癌细胞、固有层和粘膜下层中的胶原纤维，以及 脂肪组织 251 nm激发的体相紫外共振拉曼光谱 正常、腺瘤和腺癌细胞已被建模， 上述形态结构的术语。 结果将 与标准形态计量学分析的结果进行比较。

The objective of this study is understand the morphological and biochemical origin of resonance Raman signals from normal, precancerous and cancerous colon tissues. When used in reverse, we will be able to extract quantitative morphological information from tissue and predict the patholgy. So far we have studied normal and pathological samples from six patients using 251 nm UV excitation light. MicroRaman spectrometer was used to collect spectra of various mophological structures including normal cells, precancer cells, carcinoma cells, collagen fibers in lamina propria and submucosa, and adipose tissue. 251 nm excited UV resonance Raman spectra of bulk normal, adenomatous and adenocarcinoma cells have been modeled in terms of the above morphological structures. The results will be compared with those of standard morphometric analysis.