UV RESONANCE RAMAN FOR QUANTITATIVE ANALYSIS OF COLON TISSUES

用于结肠组织定量分析的紫外共振拉曼

• 批准号: 6314188
• 负责人: NADA BOUSTANY
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314188
• 关键词: biomedical equipment development; biomedical resource; human tissue; model design /development; technology /technique

We have designed and used a versatile UV resonance Raman spectrometer for quantitative analysis of cancerous in human colon tissues. The goal is to identify and quantify various chemicals in normal and diseased tissues and correlate with pathology. We have examined normal, adenomatous polyps and adenocarcinoma samples from six patients using 251 nm laser excitation. Spectra exhibit marked differences and we attribute these to increased nucleic acid to protein content in precancer and cancerous tissues compared to normal tissues. We are currently verifying these results by studying using an another laser excitation at 240 nm and using microspectroscopy.

我们设计并使用了一个多功能的紫外共振拉曼 人体结肠癌定量分析光谱仪 组织中 目标是识别和量化各种化学品， 正常和病变组织，并与病理相关。 我们有 检查了正常，腺瘤性息肉和腺癌样本， 6名患者使用251 nm激光激发。 光谱展示标记 差异，我们把这些归因于增加的核酸， 癌前组织和癌组织中的蛋白质含量与正常组织相比 组织中 我们目前正在验证这些结果， 在240 nm处的另一激光激发并使用显微光谱学。