CALCIUM SIGNALING IN THE TESTIS

睾丸中的钙信号传导

• 批准号: 6229450
• 负责人: Kathleen Mary Beckingham
• 金额: $ 19.36万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-28 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6229450
• 关键词: Drosophilidae; calcium binding protein; chimeric proteins; dynein ATPase; gene mutation; green fluorescent proteins; mutant; myosins; protein protein interaction; protein sequence; protein structure function; spermatogenesis; testis; troponin

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objectives of the proposed work are to dissect the roles of calcium regulation in sperm development and mature sperm function. The initial studies proposed here concern the role(s) of a newly identified calcium-binding protein implicated in calcium-regulatory events in the testis. The protein, termed Androcam, is specifically expressed in the testis and appears to be evolutionarily conserved from insects to mammals. The characteristics of Androcam indicate that it is a calcium sensor-a protein that converts transient changes in intracellular calcium into changes in cellular responses as a result of calcium-induced changes in its protein-protein interactions. Two major approaches to understanding Androcam function will be pursued: i) the disruptions in spermatogenesis and mature sperm function produced by mutations to the Androcam gene of Drosophila melanogaster will be analyzed; ii) the protein interaction partners of Androcam, both in the calcium-bound and calcium-free state will be identified in the same organism. Prior studies already allow the prediction that two types molecular motor proteins may be Androcam targets. The Androcam homologs in other species will also be isolated with a particular view to pursuing studies of Androcam-related proteins in mammalian species.

描述：（根据申请人的摘要进行了改编）长期目标 拟议的工作是剖析钙调节在精子中的作用 发育和成熟的精子功能。这里提出的初步研究 关注与与 睾丸中的钙调节事件。该蛋白质称为androcam，是 在睾丸中特别表达，似乎是进化保守的 从昆虫到哺乳动物。 Androcam的特征表明它是 钙传感器A蛋白转化细胞内瞬时变化 由于钙诱导的钙变为细胞反应的变化 其蛋白质蛋白质相互作用的变化。两种主要方法 了解Androcam功能将被追求：i） 突变对雄果产生的精子发生和成熟的精子功能 将分析果蝇的基因； ii）蛋白质相互作用 在钙结合和无钙状态下，Androcam的合作伙伴将是 在同一生物体中鉴定出来。先前的研究已经允许预测 这两种分子运动蛋白可能是雄性蛋白靶标。 androcam 其他物种中的同源物也将被隔离 追求哺乳动物物种中与果糖相关的蛋白质的研究。