Role of a calcium-binding protein in the saliva of Nephotettix cincticeps in ingestion from the sieve tubes

黑尾叶蝉唾液中钙结合蛋白在筛管摄入中的作用

• 批准号: 22580063
• 负责人: HATTORI Makoto
• 金额: $ 2.91万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580063/
• 关键词: 害虫管理; 唾液; EF-ハンドモチーフ; カルシウム結合タンパク質; 師管吸汁; ツマグロヨコバイ

Green rice leafhoppers (Nephotettix cincticeps) secrete saliva during feeding. In this study, we found an 84 kDa calcium-binding protein (NcSP84) in the saliva. The NcSP84 was produced in the largest lobes in the primary salivary glands. The NcSP84 was detected in the phloem sap of rice exposed to leafhoppers, verifying that the NcSP84 protein was injected into the sieve tubes. The nucleotide and amino acid sequences of NcSP84 did not share homology with any sequences in public databases. Motif search predicted that this protein had multiple EF-hands, the most common motif found in Ca^<2+>-binding proteins. As predicted, NcSP84 exhibited Ca^<2+>-binding activity. The mobility of purified NcSP84 bound to Ca^<2+> declined during SDS-PAGE, depending on the concentration of CaCl_2 present. These results suggest that NcSP84 could be secreted into the sieve tubes during feeding, which might bind Ca^<2+> ions that flow into sieve tubes in response to stylet puncturing. This might suppress sieve-element clogging and facilitate continuous ingestion from sieve tubes. This is the first finding of gene cloning of an EF-hand Ca^<2+>-binding protein in the saliva of a vascular-feeding insect

绿稻叶蝉（Nephotettix cincticeps）在进食时分泌唾液。在本研究中，我们在唾液中发现了一个84 kDa的钙结合蛋白（NcSP84）。NcSP84在初级唾液腺最大的裂片中产生。在暴露于叶蝉的水稻韧皮部液中检测到NcSP84蛋白，证实NcSP84蛋白被注射到筛管中。NcSP84的核苷酸和氨基酸序列与公开数据库中的序列无同源性。Motif搜索预测该蛋白具有多个ef -hand，这是Ca^<2+>-结合蛋白中最常见的Motif。结果表明，NcSP84具有Ca^<2+>-结合活性。在SDS-PAGE过程中，与Ca^<2+>结合的纯化NcSP84的迁移率随CaCl_2浓度的不同而下降。这些结果表明NcSP84可能在进料过程中分泌到筛管中，并可能结合在柱头刺破后流入筛管的Ca^<2+>离子。这可能会抑制筛元堵塞，并促进从筛管连续摄入。这是首次在血管食性昆虫唾液中发现EF-hand Ca^<2+>-结合蛋白的基因克隆

项目成果

Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper

绿稻叶蝉分泌唾液中新型钙结合蛋白的分子克隆

• 发表时间: 2012
• 期刊: Nephotettix cincticeps Insect Biochemistry and Molecular Biology
• 作者: Hattori M;Nakamura M;Komatsu S;Tsuchihara K;Tamura Y;Hasegawa T
• 通讯作者: Hasegawa T

長谷川毅, 服部誠, 野田博明

长谷川武、服部诚、野田宏明

• 发表时间: 2013
• 作者: 長谷川毅;服部誠;野田博明
• 通讯作者: 野田博明

A Calcium-binding and other functional proteins in the saliva of the green rice leafhopper

A 绿稻叶蝉唾液中的钙结合蛋白和其他功能蛋白

• 发表时间: 2012
• 作者: Hattori M;Komatsu S;Nakamura M;Tamura Y
• 通讯作者: Tamura Y

A calcium-binding and other functional proteins in the saliva of the green rice leafhopper, Nephotettix cincticeps

绿叶蝉 (Nephotettix cincticeps) 唾液中的钙结合蛋白和其他功能蛋白

• 发表时间: 2012
• 作者: Hattori;M.;Komatsu;S.;Nakamura,M.;Tamura;Y.
• 通讯作者: Y.

Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper Nephotettix cincticeps

• DOI: 10.1016/j.ibmb.2011.10.001
• 发表时间: 2012-01-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Hattori, Makoto;Nakamura, Masatoshi;Hasegawa, Tsuyoshi
• 通讯作者: Hasegawa, Tsuyoshi