CALCIUM SIGNALING IN THE TESTIS

睾丸中的钙信号传导

• 批准号: 6530555
• 负责人: Kathleen Mary Beckingham
• 金额: $ 19.87万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-28 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530555
• 关键词: Drosophilidae; calcium binding protein; chimeric proteins; dynein ATPase; gene mutation; green fluorescent proteins; mutant; myosins; protein protein interaction; protein sequence; protein structure function; spermatogenesis; testis; troponin

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objectives of the proposed work are to dissect the roles of calcium regulation in sperm development and mature sperm function. The initial studies proposed here concern the role(s) of a newly identified calcium-binding protein implicated in calcium-regulatory events in the testis. The protein, termed Androcam, is specifically expressed in the testis and appears to be evolutionarily conserved from insects to mammals. The characteristics of Androcam indicate that it is a calcium sensor-a protein that converts transient changes in intracellular calcium into changes in cellular responses as a result of calcium-induced changes in its protein-protein interactions. Two major approaches to understanding Androcam function will be pursued: i) the disruptions in spermatogenesis and mature sperm function produced by mutations to the Androcam gene of Drosophila melanogaster will be analyzed; ii) the protein interaction partners of Androcam, both in the calcium-bound and calcium-free state will be identified in the same organism. Prior studies already allow the prediction that two types molecular motor proteins may be Androcam targets. The Androcam homologs in other species will also be isolated with a particular view to pursuing studies of Androcam-related proteins in mammalian species.

描述：（改编自申请人的摘要）长期目标 拟议的工作是剖析精子中钙调节的作用 发育和成熟精子的功能。这里提出的初步研究 关注新发现的钙结合蛋白的作用 睾丸中的钙调节事件。这种蛋白质被称为 Androcam，是 在睾丸中特异性表达，并且似乎在进化上是保守的 从昆虫到哺乳动物。 Androcam的特性表明它是一个 钙传感器——一种转换细胞内瞬时变化的蛋白质 钙诱导的细胞反应变化 其蛋白质-蛋白质相互作用的变化。两种主要方法 理解 Androcam 功能将追求： i) 的中断 Androcam 突变产生的精子发生和成熟精子功能 将分析果蝇的基因； ii) 蛋白质相互作用 Androcam 的合作伙伴，无论是钙结合状态还是无钙状态都将是 在同一生物体中鉴定。先前的研究已经允许预测 两种类型的分子运动蛋白可能是 Androcam 的目标。安德罗卡姆 其他物种的同源物也将被分离出来，特别是为了 从事哺乳动物物种中 Androcam 相关蛋白的研究。