CORE--AMINO ACID FACILITY

核心——氨基酸设施

• 批准号: 6301034
• 负责人: PAUL J FLAKOLL
• 金额: $ 19.8万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6301034
• 关键词: alanine; aminoacid; analytical method; biomedical equipment purchase; biomedical facility; blood chemistry; branched chain aminoacid; cerebrospinal fluid; diabetes mellitus; high performance liquid chromatography; ion exchange chromatography; leucine; phenylalanine; protein sequence; protein structure; urinalysis

The Amino Acid Assay Subcore provides investigators access to a number of analytical methods related to the measurement of labelled and unlabelled amino acids. The subcore provides space, equipment and personnel for sample analysis and method development. Investigators pay a charge to cover the cost of supplies and pro-rated service contracts. The analyses available include 1) complete amino acid profiles, 2) select amino acid profiles (branched chain or gluconeogenic), 3) amino acid specific activity determinations (alanine, leucine, phenylalanine) and 4) amino acid enrichment (leucine, alpha ketoisocaproate, phenylalanine). With the exception of the rapid assay for leucine and phenylalanine, the user group identified as being associated with this subcore project an increased need for all services offered.

氨基酸分析子核心为研究者提供了许多 与标记和未标记物质测量有关的分析方法 个氨基酸次核心为以下活动提供空间、设备和人员 样品分析和方法开发。调查人员支付费用， 支付用品和按比例分摊的服务合同费用。的分析 可用的包括1）完整的氨基酸谱，2）选择的氨基酸 图谱（支链或致突变），3）氨基酸特异性 活性测定（丙氨酸、亮氨酸、苯丙氨酸）和4）氨基 酸富集（亮氨酸、α-酮异己酸、苯丙氨酸）。与 除了亮氨酸和苯丙氨酸的快速测定外， 被确定为与这一次级核心项目有关， 提供的所有服务。