EFFECTS OF SURFACE CHEMISTRY & PREADSORBED PROTEINS ON MONOCYTE ADHESION

表面化学的影响

• 批准号: 6345059
• 负责人: THOMAS Alan HORBETT
• 金额: $ 2.21万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345059
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; health care; proteins

In this study, we are investigating the effect of preadsorbed fibrinogen (Fg) and fibronectin (Fn) on monocyte and macrophage adhesion to surfaces, and cytokine secretion from the adsorbed biomolecules. The adhesion studies are done on modified 96-well polystyrene microtiter plates made of untreated polystyrene (PS), oxygenated tissue culture polystyrene (TCPS), and other commercially available plates. The surface of the plates is analyzed with ESCA. Results to date indicate that 1) monocyte adhesion and TNF( release are affected by surface chemistry and pre-adsorbed proteins, 2) the amount of TNF( released is not directly proportional to the number of adherent cells, and 3) adsorbed fibrinogen promotes the release of TNF(..

在这项研究中，我们正在研究预吸附的效果