EFFECT OF SURFACE CHEMISTRY & ADHESION PROTEINS ON PROCOAGULANT ACTIVITY: BLOOD

表面化学的影响

• 批准号: 6345061
• 负责人: THOMAS Alan HORBETT
• 金额: $ 0.49万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345061
• 关键词: animal tissue; bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; biotechnology; proteins; technology /technique

The effect of material contact and adhesion proteins on induction of the procoagulant state in platelets is being investigated. The substrates used are Immulon I, untreated 96-well microtiter plates and C3F6 RFGD plasma-coated Immulon I plates. Surfaces are preadsorbed with normal or depleted (missing Fg, Fn, or both) plasma and then exposed to the platelet-containing solution. Adherent platelets are incubated with a mixture of Va, Xa, and prothrombin in PSB. Thrombin generation is stopped at the time points of the study by the addition of EDTA. ESCA is used to characterize the composition and chemistry of the substrates.

材料接触和粘附蛋白对诱导的影响