DEVELOPMENT OF FIELD FREQUENCY LOCKS FOR L BAND SPECTROMETERS

L波段光谱仪场频锁的研制

• 批准号: 6353183
• 负责人: ILDAR SALIKOV
• 金额: $ 0.61万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353183
• 关键词: Mammalia; biological products; biomedical resource; cardiovascular system; drug /agent; gas; liver; nervous system

Despite the wealth of knowledge about the chemistry of nitric oxide (NO), there is considerable uncertainty regarding the reactions of NO in vivo. One goal of this research is to characterize the interaction of nitric oxide with protein-bound iron, typically with thiol ligands. A second goal of the research is to use dithiocarbamates as in vivo spin traps for NO in mice, allowing the real-time detection of NO through L-band EPR spectroscopy and the ex vivo analysis of individual organs through X-band spectroscopy. This technique has been successful for detecting NO production in mouse septic shock models, utilizing diethyl dithiocarbamate (DETC) and N-methyl-D-glucamine dithiocarbamate injected along with iron(II) sulfate and sodium citrate. The EPR signal observed is characteristic of a mononitrosyl iron complex; in L-band spectroscopy it consists of an essentially symmetrical triplet with splitting of approximately 12.8 Gauss due to the nitrogen. In the current trials, the signal produced by the Fe-DETC-NO complex has a greater intensity, likely due to its greater lipophilicity and ability to concentrate in cell membranes. Future work will involve testing a variety of other dithiocarbamates, including the sarcosine, pyrrolidine, and proline derivatives, in order to maximize the spectrum from trapped NO in the liver, kidney, brain, spinal cord, and in circulation.

尽管有丰富的关于硝酸化学的知识 氧化物(NO)，关于反应有相当大的不确定性 体内的一氧化氮。这项研究的一个目标是描述 一氧化氮与蛋白结合铁的相互作用，通常与 硫醇配体。这项研究的第二个目标是使用 二硫代氨基甲酸酯作为体内NO的自旋陷阱，允许 L波段电子顺磁共振光谱实时检测NO及EX 通过X波段光谱对单个器官进行活体分析。这 检测小鼠体内NO产生的技术已取得成功 利用二乙基二硫代氨基甲酸酯(DETC)和 N-甲基-D-氨基葡萄糖胺二硫代氨基甲酸酯与铁(II)一起注射 硫酸盐和柠檬酸钠。观测到的EPR信号具有特征性 指一种亚硝酸铁的络合物；在L波段光谱中，它包括 一个基本上对称的三胞胎，分裂约为 12.8Gauss是由于氮气的作用。在目前的试验中，信号 由Fe-DETC产生-没有任何络合物具有更大的强度，可能是由于 因为它具有更强的亲脂性和在细胞内浓缩的能力 膜。未来的工作将包括测试各种其他 二硫代氨基甲酸酯，包括肌氨酸、吡咯烷和脯氨酸 衍生品，以最大限度地从捕获NO的光谱中 肝、肾、脑、脊髓和循环中。