HOMOCYSTEINE VASCULAR BIOCHEMISTRY & METABOLISM

同型半胱氨酸血管生物化学

• 批准号: 6345223
• 负责人: Donald Weldon Jacobsen
• 金额: $ 0.62万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345223
• 关键词: AIDS; bacteria; biological products; biomaterials; biomedical resource; carbohydrates; immunology; infection; inflammation; lymphatic system; structural biology; virus

The intercellular matrix (NtkF) of the marine sponge, Microciona prolifera, is a large proteoglycan that is highly glycosylated, sulfated and contains equal amounts of protein and carbohydrate. There is a protein core from which radiate many arms with a high carbohydrate content. Subunits are linked to each other via carbohydrate-carbohydrate bonds, two epitopes of which have been chemically defined. Hyaluronic acid (HA) is a component of MAF, but until now itsfunctional role has been unclear. We have found that MAR as well as MbA.Fds, have potent inhibitory activity against HIV virally infected lymphoid cells and the inhibitory activity I at levels as low as 0. 1 n-&rograms/ml in the absence of any toxicity. We need to sort out, characterize and compare the components of NLAFds in order to identify those with HIV inhibitory effects. The ones which are anionic, i.e., sulfated disaccharide and sulfated tetrasaccharide, may qualify (there maybe others); since these types of anionic compounds have shown HIV inhibition. If their viral inhibitory activity is based upon charge effects, they may prove useful against other enveloped viruses with protein composition similar to HIV. Our recent studies on sponge have shown that sulfation may be an important modifier of cell functions, such as masking of lectins and modulating reactions between proteoglycans and cell surface receptors. The first batch was M[AFds or lyophilized supernatant derived from ethanolic precipitation of M[AFl (water-dialyzed purified M[AF gel that was lyophilized). The second batch was M[AFl (water-dialyzed and lyophilized purified M[AF gel), which was used to derive M[AFds. The mass spectra and GC retention times indicate five monosaccharides with the following relative abundance: Fuc 14%; Man18%; Gal 32%; GlcU 4.5%; GlcNAc; 31%. Neuraminic acid was checked by a very short time methanolysis and none was detected. The total amount of carbohydrate was calculated to be 3.7% - a very small amount.

海绵Microciona的细胞间基质（NtkF） prolifera是一种高度糖基化的大蛋白聚糖， 硫酸化，含有等量的蛋白质和碳水化合物。 有一个蛋白质核心，从那里辐射出许多高分子臂， 碳水化合物含量 子单元通过以下方式相互链接： 碳水化合物-碳水化合物键，其中的两个表位已经被 化学定义 透明质酸（HA）是MAF的一种成分，但 到目前为止，其功能作用尚不清楚。 我们发现MAR 以及MbA.Fds，对HIV具有有效的抑制活性 病毒感染的淋巴样细胞和抑制活性I， 水平低至0。 在没有任何毒性的情况下为1 μ g/ml。 我们需要整理、描述和比较NLAFds的组成部分 以鉴定那些具有HIV抑制作用的药物。 的人 它们是阴离子的，即，硫酸化双糖和硫酸化 四糖，可能合格（可能还有其他）;因为这些类型 阴离子化合物显示出HIV抑制作用。 如果病毒 抑制活性是基于电荷效应，他们可以证明 可用于对抗具有蛋白质组合物的其它包膜病毒 类似于HIV。 我们最近对海绵的研究表明， 硫酸化可能是细胞功能的重要修饰剂，例如 凝集素的掩蔽和调节蛋白聚糖与 细胞表面受体 第一批为M[AFds]或冻干 得自M[AF 1]乙醇沉淀的上清液 （水透析的纯化的M[AF]凝胶，其被冻干）。 第二 批次是M[AF]（水透析和冻干纯化的M[AF]凝胶）， 其用于导出M[AFd。 质谱和GC保留值 时间表示五种单糖，具有以下相对 丰度：Fuc 14%; Man 18%; Gal 32%; GlcU 4.5%; GlcNAc; 31%。 神经氨酸通过很短时间的甲醇分解来检测， 被发现了 碳水化合物的总量计算为 3.7%，一个很小的数字。