STRUCTURAL BASIS OF T CELL RECEPTOR RECOGNITION

T 细胞受体识别的结构基础

• 批准号: 6341688
• 负责人: IAN A WILSON
• 金额: $ 22.97万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6341688
• 关键词: CD3 molecule; CD8 molecule; MHC class I antigen; T cell receptor; T lymphocyte; X ray crystallography; antigen presentation; biological signal transduction; molecular cloning; protein structure function; tissue /cell culture

DESCRIPTION (Adapted from Investigator's abstract): The goal of this proposal is to answer a number of fundamental questions regarding the structural basis for the recognition of peptide/MHC class I complexes by T cell receptors (TCRs). Are there general structural principles which govern TCR-MHC interactions? Are these similar for TCR recognition of self and foreign MHC? Does conformational change or induced fit play a role in TCR-MHC recognition and communication among members of the TCR/MHC/CD3/CD8 signalling complex? X-ray crystallography will be used to investigate the detailed molecular interactions between a TCR specific for different syngeneic and allogeneic peptide/MHC class I complexes in the presence and absence of CD3 and CD8 components. The specific aims are to determine the structures of: (i) TCR-MHC syngeneic class I-peptide complexes, (ii) TCR-allogeneic class I-peptide complexes, (iii) TCR/MHC/CD8 ternary complexes, (iv) agonist and antagonist TCR-MHC complexes, and (v) extracellular CD3 chains and their complexes with TCR.

描述（根据调查员的摘要改编）：目的 建议是回答有关有关的许多基本问题 通过T识别肽/MHC I类配合物的结构基础 细胞受体（TCR）。 是否有一般的结构原则来管理 TCR-MHC相互作用？ 这些与TCR识别自我相似，并且 外国MHC？ 构象变化或诱发拟合是否在 TCR/MHC/CD3/CD8成员之间的TCR-MHC识别和通信 信号复合体？ X射线晶体学将用于研究 特异性的TCR之间的详细分子相互作用 在存在的同性和同种异体肽/MHC I类配合物和同种异体肽/MHC CD3和CD8组件的缺乏。 具体目的是确定 结构：（i）TCR-MHC同步I类肽络合物，（ii） TCR合成I类肽复合物，（iii）TCR/MHC/CD8三元 复合物，（IV）激动剂和拮抗剂TCR-MHC复合物，（V） 细胞外CD3链及其与TCR的复合物。