REGULATION OF HEPATOCELLULAR FUNCTION BY GROWTH HORMONE
生长激素对肝细胞功能的调节
基本信息
- 批准号:6350648
- 负责人:
- 金额:$ 23.09万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-05-01 至 2003-01-31
- 项目状态:已结题
- 来源:
- 关键词:affinity chromatography biological signal transduction corticosteroid receptors gene expression gene induction /repression genetic promoter element genetic regulatory element hormone regulation /control mechanism immunoprecipitation interleukin 6 laboratory rat liver cells protease inhibitor serine proteinases somatotropin tissue /cell culture transcription factor transfection
项目摘要
DESCRIPTION: This proposal addresses the molecular basis of growth hormone
action in the liver through examination of the rat Spi I (serine protease
inhibitor) 2 locus. Three hypotheses will be tested. The first is that
regulation of the Stat 2.1 gene is mediated by binding of Stat5 in a
cooperative fashion to two sites within the GHRE. The second is that
additional proteins interact with Stat5 to mediate regulation. The third is
that specificity of GH regulation through the Stat5 pathway is achieved
through the unique architecture of the GHRE in the Spi 2.1 gene and these
Stat5-protein interactions. Three specific aims are proposed to test these
hypotheses. 1. The investigator will further define the nuclear proteins
critical for GH action in the Spi 2.1 model, establishing proof that Stat5
is necessary for Spi 2.1 gene activation, examining the role of YY1 in GH
action, determining the role of glucocorticoid receptor, and evaluating the
role of serine phosphorylation of Stat5. 2. The investigator will examine
the functional architecture of the GHRE, exploring the significance of
paired GAS-like elements in the response to GH, distinguishing
characteristics of DNA elements structurally related to the Spi 2.1 GHRE but
not responsive to GH, and defining other elements in the Spi 2.1 promoter
important in the response to GH. 3. The investigator will compare the
actions of GH and IL-6 in Spi 2.1 gene expression, defining the IL-6
response element and the nuclear factors binding to Spi 2.1 in response to
IL-6 with comparison to the GH response, thereby furthering understanding of
the specificity of the Spi 2.1 response to GH.
描述:该提案涉及生长激素的分子基础。
大鼠SPI I(丝氨酸蛋白酶)在肝脏中的作用
抑制物)2个基因座。三个假说将被检验。第一个是
Stat 2.1基因的调节是由Stat5结合介导的。
在GHRE内的两个地点的合作方式。第二个就是
更多的蛋白质与Stat5相互作用,介导调节。第三个是
通过Stat5途径实现GH调节的特异性
通过SPI2.1基因中GHRE的独特结构和这些
STAT5-蛋白质相互作用。提出了三个具体的目标来测试这些
假设。1.调查员将进一步定义核蛋白
对SPI 2.1模型中的GH作用至关重要,证明Stat5
是SPI2.1基因激活所必需的,检测YY1在生长激素中的作用
作用,确定糖皮质激素受体的作用,并评估
STAT5丝氨酸磷酸化的作用。2.调查员将审查
GHRE的功能架构,探讨了
成对的类气体元素对生长激素的响应,区分
结构上与SPI 2.1 GHRE相关的DNA元件的特征
不响应GH,并定义SPI 2.1启动子中的其他元件
在对生长激素的反应中很重要。3.调查员将比较
GH和IL-6在定义IL-6的SPI2.1基因表达中的作用
响应元件和结合SPI 2.1的核因子对
IL-6与生长激素反应的比较,从而加深对
SPI2.1对GH反应的特异性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Susan A. Berry其他文献
Infant with multiple congenital anomalies and deletion (9)(q34.3).
患有多种先天性异常和缺失的婴儿 (9)(q34.3)。
- DOI:
- 发表时间:
1994 - 期刊:
- 影响因子:0
- 作者:
Lisa A. Schimmenti;Susan A. Berry;Mendel Tuchman;Betsy A. Hirsch - 通讯作者:
Betsy A. Hirsch
Ontogeny and pituitary regulation of testicular growth hormone-releasing hormone-like messenger ribonucleic acid.
睾丸生长激素释放激素样信使核糖核酸的个体发育和垂体调节。
- DOI:
- 发表时间:
1990 - 期刊:
- 影响因子:4.8
- 作者:
Susan A. Berry;O. Pescovitz - 通讯作者:
O. Pescovitz
Phenylalanine hydroxylase deficiency diagnosis and management: A 2023 evidence-based clinical guideline of the American College of Medical Genetics and Genomics (ACMG)
苯丙氨酸羟化酶缺乏症的诊断和管理:美国医学遗传学和基因组学学院(ACMG)2023 年基于证据的临床指南
- DOI:
10.1016/j.gim.2024.101289 - 发表时间:
2025-01-01 - 期刊:
- 影响因子:6.200
- 作者:
Wendy E. Smith;Susan A. Berry;Kaitlyn Bloom;Christine Brown;Barbara K. Burton;Olivia M. Demarest;Gabrielle P. Jenkins;Jennifer Malinowski;Kim L. McBride;H. Joel Mroczkowski;Curt Scharfe;Jerry Vockley;ACMG Board of Directors - 通讯作者:
ACMG Board of Directors
Understanding patient, caregiver, and healthcare provider perspectives of the management of long-chain fatty acid oxidation disorders
了解患者、护理人员和医疗保健提供者对长链脂肪酸氧化障碍管理的看法
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Eileen Sullivan Baker;Jennifer Botham;Tasia Rechisky;Evelyn Romano;Daniel Garcia;Susan A. Berry - 通讯作者:
Susan A. Berry
INCREASED PLACENTAL IRON-RESPONSIVE PROTEIN-1 (IRP-1) AND TRANSFERRIN RECEPTOR (TfR) mRNA IN DIABETIC PREGNANCIES COMPLICATED BY FETAL IRON DEFICIENCY † 248
糖尿病合并胎儿缺铁性贫血的孕妇胎盘铁反应蛋白-1(IRP-1)和转铁蛋白受体(TfR)mRNA 表达增加†248
- DOI:
10.1203/00006450-199704001-00268 - 发表时间:
1997-04-01 - 期刊:
- 影响因子:3.100
- 作者:
Michael K. Georgieff;Elizabeth A. Liebold;Jane D. Wobken;Susan A. Berry - 通讯作者:
Susan A. Berry
Susan A. Berry的其他文献
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{{ truncateString('Susan A. Berry', 18)}}的其他基金
Collaborative Defining the Natural History of Inborn errors of Metabolism
协作定义先天性代谢错误的自然史
- 批准号:
8121229 - 财政年份:2011
- 资助金额:
$ 23.09万 - 项目类别:
Collaborative Defining the Natural History of Inborn errors of Metabolism
协作定义先天性代谢错误的自然史
- 批准号:
8437224 - 财政年份:2011
- 资助金额:
$ 23.09万 - 项目类别:
Collaborative Defining the Natural History of Inborn errors of Metabolism
协作定义先天性代谢错误的自然史
- 批准号:
8255564 - 财政年份:2011
- 资助金额:
$ 23.09万 - 项目类别:
Collaborative Defining the Natural History of Inborn errors of Metabolism
协作定义先天性代谢错误的自然史
- 批准号:
8615922 - 财政年份:2011
- 资助金额:
$ 23.09万 - 项目类别:
Collaborative Defining the Natural History of Inborn errors of Metabolism
协作定义先天性代谢错误的自然史
- 批准号:
8813605 - 财政年份:2011
- 资助金额:
$ 23.09万 - 项目类别:
REGULATION OF HEPATOCELLULAR FUNCTION BY GROWTH HORMONE
生长激素对肝细胞功能的调节
- 批准号:
6150625 - 财政年份:1998
- 资助金额:
$ 23.09万 - 项目类别:
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