Force microscopy with arbitrary optically-trapped probes and application to internal mechanics of cells

具有任意光捕获探针的力显微镜及其在细胞内部力学中的应用

• 批准号: DP140100753
• 负责人: Prof Halina Rubinsztein-Dunlop
• 金额: $ 31.46万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140100753
• 关键词: Force; microscopy; arbitrary; optically; trapped

The ability to perform micromanipulation on particles, macromolecules, subcellular organelles, or whole cells is fundamental in elucidating processes such as chromosome movement during cell division, and movement of cell components in and out of the cell. The recent advances in optical tweezers have allowed this type of micromanipulation to approach reality. However, determination of the true optical force is critical for this technique to reach its full potential. This project will develop novel techniques to quantitatively determine the absolute optical force applied to the cell component using the process of ingestion (phagocytosis) as a proof-of-principle test, and measure forces in chromosome movement and vesicle transport within cells.

对颗粒、大分子、亚细胞器或整个细胞进行显微操作的能力是阐明细胞分裂过程中染色体运动以及细胞组分进出细胞等过程的基础。光镊的最新进展使这种类型的显微操作接近现实。然而，确定真正的光学力是这项技术发挥其全部潜力的关键。本项目将开发新技术，以摄取过程（吞噬作用）作为原理验证试验，定量测定施加于细胞成分的绝对光学力，并测量细胞内染色体运动和囊泡运输的力。