Modulation of MUC1 Mucin Trafficking

MUC1 粘蛋白运输的调节

• 批准号: 6327141
• 负责人: Rebecca P Hughey
• 金额: $ 29.33万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6327141
• 关键词: CHO cells; MDCK cell; biomarker; endocytosis; enzyme inhibitors; fatty acylation; gene expression; glycoprotein biosynthesis; glycoproteins; glycosylation; hexosyltransferase; immunoprecipitation; intracellular transport; isomerase; metastasis; molecular pathology; molecular site; mucins; palmitates; phosphorylation; protein localization; protein structure function; proteolysis; radiotracer; site directed mutagenesis; western blottings

DESCRIPTION (applicant's description): MUC1 is a well characterized marker for many cancers of epithelial origin that also directly contributes to the aggressive metastatic phenotype of tumors. Cumulative data from studies of these cancer patients indicates that high levels of particularly intracellularMUC1 in the tumor and in serum correlates with a strong potential for metastasis and a poor prognosis for the patient. The anti-adhesive property of this normally apical, transmembrane mucin (with 40-90 heavily 0-glycosylated tandem repeats of 20 aa) disrupts both cell-cell and cell-matrix interactions in non-polarized tumor cells, thus enhancing metastasis. The soluble MUC1 in patient serum and ascites also contributes to the aggressive phenotype of the cancer by acting as a tumor decoy by forming immunocomplexes with antibodies, by binding T-cells and blocking their proliferation, and by rebinding to the tumor and initiating signal transduction pathways. Since MUC1 has fewer and smaller 0-glycans in many tumors, preliminary studies were designed to understand how this contributes to the intracellular accumulation and shedding of MUC1 since the mechanisms for either event is unknown. Using normal and glycosylation-defective CHO cells, metabolic labeling and biotinylation, we find that cell surface expression of recombinant human MUC1 is dependent on 0-glycosylation. Surprisingly, we find that this enormous molecule is internalized by clathrin-mediated endocytosis indicating that the 40-90 tandem-repeat immunodominanat epitopes of MUCI are an ideal target for immunotoxin therapy. Thus, it is essential to understand what regulates MUC1 endocytosis and shedding to better target treatments to MUC1-positive tumors and avoid soluble MUCI in serum/ascites. We find that MUC1 endocytosis is blocked by mutation of a potential site for dual palmitoylation and enhanced by smaller 0-glycans, indicating that its trafficking may be modulated by homotypic clustering and association with lipid microdomains involved in signal transduction. Since we also find that MUCI shedding is endocytosis-dependent, our specific aims are designed to understand how MUCI trafficking and shedding is modulated by its 0-glycan structure, palmitoylation, and phosphorylation. Initial experiments will utilize non-polarized CHO cells while later experiments in new glycosylation-defective MDCK cells will define apical-specific modulation of MUCI trafficking.

描述（申请人描述）：MUC1是一种表征良好的标记物