TRIPLE QUADRUPOLE MASS SPECTROMETER

一个由七名NIH资助的研究人员组成的小组要求提供资金，以购买一台带有电喷雾电离和大气压化学电离的三重四极质谱仪，并与一台液相色谱仪相连。该仪器将为华盛顿州立大学的核心小组和其他生物分子研究人员提供各种灵敏和选择性定量LC/MS分析、用于结构解析的串联质谱和一般LC/MS检测的能力。该仪器将位于生物分析和生物技术实验室，2号单元，WSU的中央MS设施。该仪器支持的研究项目包括P450介导反应产物的定量和表征;催化萜类底物环化的酶和突变酶的表征;精子发生过程中体细胞和生殖细胞中的基因表达;生物活性胞壁酰肽的结构表征;人类蛋白质A.T特异性DNA结合特性的研究;以及氧化应激的异前列烷生物标志物的定量和鉴定。

Funds to purchase a triple quadrupole mass spectrometer with electrospray ionization and atmospheric pressure chemical ionization, coupled to a liquid chromatograph, is requested by a group of seven NIH funded investigators. The instrument will provide capability for a variety of sensitive and selective quantitative LC/MS analyses, tandem mass spectrometry for structure elucidation, and general LC/MS detection for the core group and other biomolecular researchers at Washington State University. The instrument will be located in the Laboratory for Bioanalysis and Biotechnology, Unit 2, WSU's central MS facility. Research projects to be supported by this instrument include quantitation and characterization of products from P450 mediated reactions; characterization of enzymes and mutant enzymes which catalyze the cyclization of terpenoid substrates; gene expression in somatic and germ cells during spermatogenesis; structural characterization of biologically active muramyl peptides; investigation of A.T specific DNA-binding properties of a human protein; and quantitation and identification of isoprostane biomarkers of oxidative stress.