PHILIPS TECNAI 12 ELECTRON MICROSCOPE

飞利浦 TECNAI 12 电子显微镜

• 批准号: 6288116
• 负责人: FREDERICK NAFTOLIN
• 金额: $ 38.79万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288116
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; female reproductive system; transmission electron microscopy

This application responds to NIH program announcement PAR-99-031 (NCRR shared instrumentation grant) and requests funding to purchase a replacement transmission electron microscope for the Department of Obstetrics and Gynecology, Yale University School of Medicine. For the last three decades, under NIH support, the reproductive neuroscience group in our department has been studying basic central and peripheral mechanisms that underlie normal reproduction and pathological conditions, primarily using morphological methods. We have also been collaborating with other departments. The transmission electron microscope is used for funded projects in the department (80%) plus collaborative projects (20%). The 12 projects listed in the application use ultrastructural analyses by transmission electron microscopy. Through a similar NIH initiative, we purchased a Philips CM 10 electron microscope 15 years ago. The Department has paid for the construction and maintenance of the electron microscopic suite and supporting facilities during this period, and will continue to do so. Although there are other electron microscopes in other departments of the Medical School and the University, because all the projects of the PI and those of Drs. Csaba Leranth and Tamas Horvath utilize electron microscopy as a primary tool of investigation our group has the need for the equipment almost every hour of the working day and on weekends. Thus, it is impossible to resolve our need of an electron microscope on a shared basis with other departments. Through this initiative, we are applying to purchase a new electron microscope: Our 15 year old Philips CM 10 transmission electron microscope contributed observations that have been published in hundreds of peer reviewed research articles, but it is now completely broken and out of service. The heavy use of the machine resulted in increasing numbers of breakdowns during the past year. Because this model has been long discontinued, it is difficult to find the appropriate replacement parts to rebuild the microscope. It has been abandoned as a viable instrument and we are temporarily using a "mothballed" obsolete machine. But, this one cannot withstand the load and will soon break down for good. Twelve government-supported projects are listed (see text) that in the aggregate require constant use of an electron microscope. Without the possibility of purchasing a new electron microscope, these projects are in jeopardy! It is the conclusion of all participants that the most user-friendly and durable microscope currently available is the Philips Tecnai 10, which is the model that replaced the popular Philips CM 100 model. We describe the Philips Tecnai 10 in the text. We are requesting funds urgently to purchase this machine and an image analysis package in order to support our NIH-funded projects.

本申请响应NIH项目公告PAR-99-031 （NCRR共享仪器拨款），请求资助为耶鲁大学医学院妇产科购买一台替代的透射电子显微镜。在过去的三十年里，在美国国立卫生研究院的支持下，我们系的生殖神经科学小组一直在研究正常生殖和病理条件下的基本中枢和外周机制，主要使用形态学方法。我们也一直在与其他部门合作。透射电镜用于本系资助项目（80%）和合作项目（20%）。应用程序中列出的12个项目使用透射电子显微镜进行超微结构分析。通过类似的NIH倡议，我们在15年前购买了一台飞利浦CM - 10电子显微镜。在此期间，该部已支付建造和维修电子显微镜室和支助设施的费用，并将继续这样做。虽然在医学院和大学的其他部门也有其他的电子显微镜，但由于PI和博士的所有项目。Csaba Leranth和Tamas Horvath利用电子显微镜作为主要的调查工具，我们的团队几乎每个工作日和周末都需要设备。因此，在与其他部门共享的基础上解决我们对电子显微镜的需求是不可能的。通过这一举措，我们申请购买一台新的电子显微镜：我们使用了15年的飞利浦CM 10透射电子显微镜，发表了数百篇同行评议的研究文章，但现在它完全坏了，不能使用了。在过去的一年里，这台机器的大量使用导致了越来越多的故障。因为这个型号已经停产很久了，很难找到合适的替换零件来重建显微镜。它作为一种可行的仪器已经被抛弃了，我们暂时使用的是一台“封存”的过时机器。但是，这个人无法承受这种负荷，很快就会永远崩溃。这里列出了12个政府支持的项目（见原文），这些项目总共需要经常使用电子显微镜。如果没有购买新的电子显微镜的可能，这些项目将处于危险之中！所有参与者的结论是，目前可用的最用户友好和耐用的显微镜是飞利浦Tecnai 10，这是取代流行的飞利浦CM 100模型的模型。我们在文中描述了飞利浦Tecnai 10。我们迫切要求资金购买这台机器和一个图像分析包，以支持我们的nih资助的项目。