PHILIPS TECNAI 12 ELECTRON MICROSCOPE

本申请是对NIH项目公告PAR-99-031（NCRR共享仪器补助金）的回应，并要求提供资金为耶鲁大学医学院妇产科系购买一台替换透射电子显微镜。在过去的三十年里，在美国国立卫生研究院的支持下，我们系的生殖神经科学小组一直在研究正常生殖和病理条件下的基本中枢和外周机制，主要使用形态学方法。我们还与其他部门合作。透射电子显微镜用于部门资助项目（80%）和合作项目（20%）。申请中列出的12个项目使用透射电子显微镜进行超微结构分析。15年前，通过类似的NIH计划，我们购买了一台Philips CM 10电子显微镜。该部在此期间支付了电子显微镜室和辅助设施的建造和维修费用，并将继续这样做。虽然在医学院和大学的其他部门也有其他的电子显微镜，但由于PI的所有项目以及Csaba Leranth和Tamas Horvath博士的项目都将电子显微镜作为主要的调查工具，我们小组几乎在工作日的每个小时和周末都需要设备。因此，不可能解决我们与其他部门共享电子显微镜的需求。通过这一举措，我们正在申请购买一台新的电子显微镜：我们使用了15年的Philips CM 10透射电子显微镜提供了数百篇同行评审的研究文章中发表的观察结果，但现在它已经完全坏了。去年机器的大量使用导致故障次数增加。由于这种型号已经停产很久，很难找到合适的替换零件来重建显微镜。它已被放弃作为一个可行的工具，我们暂时使用一个“封存”的过时机器。但是，这一个不能承受的负荷，很快就会坏了。列出了12个政府支持的项目（见正文），这些项目总体上需要不断使用电子显微镜。没有购买新的电子显微镜的可能性，这些项目处于危险之中！所有与会者的结论是，目前可用的最方便用户和最耐用的显微镜是Philips Tecnai 10，这是取代流行的Philips CM 100型号的型号。我们在文中描述了Philips Tecnai 10。我们正在紧急申请资金购买这台机器和一个图像分析软件包，以支持我们的NIH资助的项目。

This application responds to NIH program announcement PAR-99-031 (NCRR shared instrumentation grant) and requests funding to purchase a replacement transmission electron microscope for the Department of Obstetrics and Gynecology, Yale University School of Medicine. For the last three decades, under NIH support, the reproductive neuroscience group in our department has been studying basic central and peripheral mechanisms that underlie normal reproduction and pathological conditions, primarily using morphological methods. We have also been collaborating with other departments. The transmission electron microscope is used for funded projects in the department (80%) plus collaborative projects (20%). The 12 projects listed in the application use ultrastructural analyses by transmission electron microscopy. Through a similar NIH initiative, we purchased a Philips CM 10 electron microscope 15 years ago. The Department has paid for the construction and maintenance of the electron microscopic suite and supporting facilities during this period, and will continue to do so. Although there are other electron microscopes in other departments of the Medical School and the University, because all the projects of the PI and those of Drs. Csaba Leranth and Tamas Horvath utilize electron microscopy as a primary tool of investigation our group has the need for the equipment almost every hour of the working day and on weekends. Thus, it is impossible to resolve our need of an electron microscope on a shared basis with other departments. Through this initiative, we are applying to purchase a new electron microscope: Our 15 year old Philips CM 10 transmission electron microscope contributed observations that have been published in hundreds of peer reviewed research articles, but it is now completely broken and out of service. The heavy use of the machine resulted in increasing numbers of breakdowns during the past year. Because this model has been long discontinued, it is difficult to find the appropriate replacement parts to rebuild the microscope. It has been abandoned as a viable instrument and we are temporarily using a "mothballed" obsolete machine. But, this one cannot withstand the load and will soon break down for good. Twelve government-supported projects are listed (see text) that in the aggregate require constant use of an electron microscope. Without the possibility of purchasing a new electron microscope, these projects are in jeopardy! It is the conclusion of all participants that the most user-friendly and durable microscope currently available is the Philips Tecnai 10, which is the model that replaced the popular Philips CM 100 model. We describe the Philips Tecnai 10 in the text. We are requesting funds urgently to purchase this machine and an image analysis package in order to support our NIH-funded projects.