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PURCHASE OF A 600 MHZ NMR SPECTROMETER

购买 600 MHZ 核磁共振波谱仪

基本信息

批准号：
6294369
负责人：
John SantaLucia
金额：
$ 47万
依托单位：
WAYNE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-04-01 至 2003-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6294369
关键词：
biomedical equipment purchase nuclear magnetic resonance spectroscopy structural biology

项目摘要

The goal of this proposal is to purchase a 600 MHz NMR spectrometer to enhance ongoing research at Wayne State University in the areas of A) ribosomal RNA structure and function, B) RNA-protein and RNA-antibiotic complex structure, C) DNA-carcinogen adduct structure, D) protein structure determination of alpha-1,3- galactosyltransferase and proteins involved in metal transport and resistance, and E) assay of metabolite concentrations in neuronal cell lysates. These topics represent the research of six well-established NIH-funded laboratories. At least 85% of the time on the new 600 MHz NMR will be devoted to these research projects. The remaining instrument time will be devoted to other active areas of research at Wayne State University including: the structure of proteins with bound peptide mimics, polymer chemistry, natural products chemistry, mechanistic organic chemistry, and RNase III-RNA interactions. The only instrument at Wayne State University that is suitable for studying biomolecules is a Varian UNITY 500 MHz NMR. The console on this instrument is obsolete and it cannot run several modern pulse sequences. In addition, only half of the time on this instrument is available for studies of biomolecules. The addition of a 600 MHz NMR will significantly extend our NMR capabilities and will meet the growing demand at Wayne State University for time on a high-field NMR instrument. The increased field strength and improved console and probe design will result in a approximate 30-40% improvement in signal-to-noise ratio, will reduce spectral artifacts, and will improve spectral resolution by 20% in each dimension (44% improvement in 2D-NMR and 73% improvement in 3D-NMR). The improve performance of a 600 MHz NMR spectrometer and availability of more instrument time will increase both the quality and quantity of NMR data. This in turn will extend our ability to study the structures of larger biomolecules. Due to the large increase in the number of groups at Wayne State University performing biomolecular NMR, a rigorous training program has been initiated. New users are trained by Dr. Ksebati on the proper use of NMR instrumentation. Dr. SantaLucia holds weekly meetings on the theory and practice of NMR, which are consistently attended by more than 20 students and postdoctoral fellows. Students are trained from elementary NMR through advanced theory, including density matrix and product operator descriptions of NMR and pulse sequence programming.

该提案的目标是购买一台 600 MHz NMR 波谱仪，以增强韦恩州立大学正在进行的 A) 核糖体 RNA 领域的研究结构和功能，B) RNA-蛋白质和 RNA-抗生素复合物结构，C) DNA-致癌物加合物结构，D) α-1,3-蛋白质结构测定半乳糖基转移酶和参与金属转运和抵抗的蛋白质，以及 E) 神经元细胞裂解物中代谢物浓度的测定。这些话题代表 NIH 资助的六个知名实验室的研究。至少新 600 MHz NMR 上 85% 的时间将用于这些研究项目。剩余的仪器时间将用于其他活跃领域韦恩州立大学的研究包括：结合蛋白质的结构肽模拟物、高分子化学、天然产物化学、机理有机化学和 RNase III-RNA 相互作用。韦恩州立大学唯一适合学习的仪器 biomolecules 是瓦里安 UNITY 500 MHz NMR。本仪器上的控制台已过时，无法运行多个现代脉冲序列。另外，只有一半该仪器上的时间可用于生物分子的研究。补充 600 MHz NMR 将显着扩展我们的 NMR 能力，并将满足韦恩州立大学对高场核磁共振时间的需求不断增长乐器。增强的场强以及改进的控制台和探头设计将导致信噪比大约提高 30-40%，减少光谱伪影，并将每个光谱分辨率提高 20% 尺寸（2D-NMR 提高了 44%，3D-NMR 提高了 73%）。提高 600 MHz NMR 波谱仪的性能和可用性更多的仪器时间将提高 NMR 数据的质量和数量。这反过来将扩展我们研究更大生物分子结构的能力。由于韦恩州立大学团体数量大幅增加为了进行生物分子核磁共振，一项严格的培训计划已经启动。 Ksebati 博士对新用户进行有关正确使用 NMR 仪器的培训。 SantaLucia 博士每周召开一次有关 NMR 理论和实践的会议，持续有20多名学生和博士后参加。学生接受从初级 NMR 到高级理论的培训，包括 NMR 和脉冲序列的密度矩阵和乘积算子描述编程。