CORE--STEM CELL AND TISSUE CULTURE

核心——干细胞和组织培养

• 批准号: 6358988
• 负责人: PETER J. QUESENBERRY
• 金额: $ 15.38万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-29 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6358988
• 关键词: biomedical facility; bone marrow transplantation; flow cytometry; hematopoietic stem cells; tissue /cell culture

The Stem Cell and Tissue Culture Core will provide a centralized facility and capacity for cell line and hematopoietic stem cell purification, synchronization and culture for all participants in the Program Project. Specifically, the Core will provide a focused expertise in the maintenance of cells lines and a major emphasis on the purification of murine hematopoietic stem/progenitor cells. These purifications will be based on cell surface expression of phenotypic markers and the utilization of low staining with Rhodamine and Hoechst to define primitive quiescent cells. Purification procedures will make extensive use of the current fluorescent activated cell sorting core (BD FACS Star plus) and of a new research dedicated cell sorting core currently under development which will feature the MoFlo high speed cell sorter from Cytomation. This latter core will allow us to proceed with more developmental work with regard to cell sorting and will allow for higher speed sorting and experiments at all hours of the day and night. Cores will also develop and refine protocols for progenitor stem cell synchronization and transplantation. By consolidating cell culture activities in a single source the core will function ot standardized cell populations used in parallel by the various projects and will be able to provide individual projects with a uniform quality and consistent supply of cytokines and culture reagents. A critical feature of the core will be the provision of highly purified progenitor/stem cell populations to each of the projects and the development of new transplantation models.

干细胞和组织培养中心将提供一个集中的设施， 以及细胞系和造血干细胞纯化能力， 为计划项目的所有参与者提供同步和文化。 具体而言，核心将提供集中的专业知识， 的细胞系和主要重点是纯化鼠 造血干/祖细胞。 这些净化将基于 表型标记的细胞表面表达和低利用率 用罗丹明和Hoechst染色以确定原始静止细胞。 纯化程序将广泛使用目前的荧光 活化细胞分选核心（BD FACS星星plus）和一项新的研究 目前正在开发的专用细胞分选核心， Cytomation的MoFlo高速细胞分选仪。 后一个核心将 使我们能够继续进行更多的关于细胞的开发工作， 排序，并将允许更高的速度排序和实验在所有 白天和黑夜的时间。 核心还将制定和完善协议 用于祖细胞同步化和移植。 通过 将细胞培养活动整合到单一来源中， 标准化细胞群的功能， 项目，并将能够提供统一的个人项目 细胞因子和培养试剂的质量和稳定供应。 一 核心的关键特征将是提供高度纯化的 祖细胞/干细胞群体的每一个项目和 开发新的移植模型。