CORE--TRANSGENIC ANIMALS

核心——转基因动物

• 批准号: 6302270
• 负责人: DAVID B AVERILL
• 金额: $ 17.94万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302270
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse; laboratory rat

Description The primary aim of this Core is to coordinate the use of animals needed by investigators of each of the five projects of this Program Project. Activities include 1) the procurement of animals either from commercial vendors or the production of (mRen-2)27 and Hannover SD (SD) rats from breeder animals maintained by this core facility, 2) the maintenance of a homozygous (mRen-2)27 transgenic rat colony and SD rat colony, 3) the coordination of housing provided by the Animal Resources Program (ARP) at the Wake Forest University School of Medicine of all animals used by investigators of this Program Project grant, and 4) to assist investigators in the preparation of animals for experiments proposed in this Program Project grant. Finally, Program Project investigators may consult with Drs. Averill (Project Leader) and Strawn (Project Co- investigator) in the choice of other animal models as the need arises based upon the outcome of studies in this Program Project grant. The experiments described in this Program Project will, in part, use hemizygous (mRen-2)27 transgenic or Han SD rats. Hemizygous (mRen-2)27 transgenic rats are offspring of a homozygous (mRen-2)27 male rat mated with a female SD rat. These hemizygous (mRen-2)27 transgenic hypertensive rats are referred to as production hemizygous animals (TG*). It is estimated that the animal facility associated with the Core will deliver approximately 325 Tg+ and 350 SD rats per year to Program Project investigators during the proposed 5 year term of this Program Project renewal. In addition, the Core will coordinate the use for the approximately 145 SHR and 140 WKY rats which will be used yearly by Program Project investigators.

描述 该核心的主要目的是协调所需动物的使用 由该计划项目的五个项目中每一个项目的研究人员制定。 活动包括 1) 从商业机构采购动物 供应商或 (mRen-2)27 和汉诺威 SD (SD) 大鼠的生产 该核心设施饲养的种畜，2) 纯合 (mRen-2)27 转基因大鼠群体和 SD 大鼠群体，3) 协调动物资源计划 (ARP) 提供的住房 维克森林大学医学院使用的所有动物 本计划项目资助的研究人员，以及 4) 协助 研究人员为实验中提出的动物做准备 本计划项目拨款。最后，计划项目研究人员可能 咨询医生。 Averill（项目负责人）和 Strawn（项目合作者） 研究者）根据需要选择其他动物模型 基于本计划项目拨款的研究结果。 本计划项目中描述的实验将部分使用 半合子 (mRen-2)27 转基因或汉族 SD 大鼠。半合子 (mRen-2)27 转基因大鼠是纯合 (mRen-2)27 雄性大鼠交配的后代 与一只雌性 SD 大鼠。这些半合子 (mRen-2)27 转基因 高血压大鼠被称为生产半合子动物 （TG*）。据估计，与核心相关的动物设施 每年将为该计划提供约 325 只 Tg+ 和 350 只 SD 大鼠 本计划拟议 5 年期限内的项目调查员 项目更新。此外，核心将协调使用 每年将使用约 145 只 SHR 和 140 只 WKY 大鼠 计划 项目调查员。