CORE--TRANSGENIC ANIMALS

描述 这个核心的主要目标是协调所需动物的使用 由本计划项目的五个项目中的每个项目的调查人员进行。 活动包括1)从商业机构采购动物 供应商或生产(MREN-2)27和汉诺威SD(SD)大鼠 由该核心设施维护的繁育动物，2)维护 纯合子(MREN-2)27转基因大鼠群体和SD大鼠群体，3) 协调动物资源计划(ARP)提供的住房 在维克森林大学医学院使用的所有动物 本计划项目拨款的调查人员，以及4)协助 在为实验准备动物方面的研究人员 本计划项目拨款。最后，计划项目调查员可以 咨询Averill博士(项目负责人)和Strawn博士(项目合作伙伴) 调查员)在需要时选择其他动物模型 根据本计划项目资助的研究结果。 本项目中描述的实验将在一定程度上使用 半合子(MREN-2)转基因或汉族SD大鼠27只。半合子(MREN-2)27 转基因大鼠是纯合(MREN-2)27只雄性大鼠交配的后代 和一只雌性SD大鼠。这些半合子(MREN-2)27转基因 高血压大鼠被称为产卵半合子动物 (Tg*)。据估计，与核心相关的动物设施 每年将向计划提供约325只TG+和350只SD大鼠 本计划拟议的5年任期内的项目调查员 项目续签。此外，核心将协调使用 每年将使用大约145只SHR和140只WKY大鼠 计划项目调查员。

Description The primary aim of this Core is to coordinate the use of animals needed by investigators of each of the five projects of this Program Project. Activities include 1) the procurement of animals either from commercial vendors or the production of (mRen-2)27 and Hannover SD (SD) rats from breeder animals maintained by this core facility, 2) the maintenance of a homozygous (mRen-2)27 transgenic rat colony and SD rat colony, 3) the coordination of housing provided by the Animal Resources Program (ARP) at the Wake Forest University School of Medicine of all animals used by investigators of this Program Project grant, and 4) to assist investigators in the preparation of animals for experiments proposed in this Program Project grant. Finally, Program Project investigators may consult with Drs. Averill (Project Leader) and Strawn (Project Co- investigator) in the choice of other animal models as the need arises based upon the outcome of studies in this Program Project grant. The experiments described in this Program Project will, in part, use hemizygous (mRen-2)27 transgenic or Han SD rats. Hemizygous (mRen-2)27 transgenic rats are offspring of a homozygous (mRen-2)27 male rat mated with a female SD rat. These hemizygous (mRen-2)27 transgenic hypertensive rats are referred to as production hemizygous animals (TG*). It is estimated that the animal facility associated with the Core will deliver approximately 325 Tg+ and 350 SD rats per year to Program Project investigators during the proposed 5 year term of this Program Project renewal. In addition, the Core will coordinate the use for the approximately 145 SHR and 140 WKY rats which will be used yearly by Program Project investigators.