CORE--TRANSGENIC ANIMALS

核心——转基因动物

• 批准号: 6434957
• 负责人: DAVID B AVERILL
• 金额: $ 13.19万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6434957
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse; laboratory rat

Description The primary aim of this Core is to coordinate the use of animals needed by investigators of each of the five projects of this Program Project. Activities include 1) the procurement of animals either from commercial vendors or the production of (mRen-2)27 and Hannover SD (SD) rats from breeder animals maintained by this core facility, 2) the maintenance of a homozygous (mRen-2)27 transgenic rat colony and SD rat colony, 3) the coordination of housing provided by the Animal Resources Program (ARP) at the Wake Forest University School of Medicine of all animals used by investigators of this Program Project grant, and 4) to assist investigators in the preparation of animals for experiments proposed in this Program Project grant. Finally, Program Project investigators may consult with Drs. Averill (Project Leader) and Strawn (Project Co- investigator) in the choice of other animal models as the need arises based upon the outcome of studies in this Program Project grant. The experiments described in this Program Project will, in part, use hemizygous (mRen-2)27 transgenic or Han SD rats. Hemizygous (mRen-2)27 transgenic rats are offspring of a homozygous (mRen-2)27 male rat mated with a female SD rat. These hemizygous (mRen-2)27 transgenic hypertensive rats are referred to as production hemizygous animals (TG*). It is estimated that the animal facility associated with the Core will deliver approximately 325 Tg+ and 350 SD rats per year to Program Project investigators during the proposed 5 year term of this Program Project renewal. In addition, the Core will coordinate the use for the approximately 145 SHR and 140 WKY rats which will be used yearly by Program Project investigators.

描述 该核心的主要目的是协调所需动物的使用， 由本计划项目的五个项目中的每一个项目的调查人员进行。 活动包括1）从商业动物采购 供应商或（mRen-2）27和汉诺威SD（SD）大鼠的生产 由该核心设施饲养的繁殖动物，2） 纯合（mRen-2）27转基因大鼠群和SD大鼠群，3） 协调动物资源计划（阿普）提供的住房 在维克森林大学医学院， 该计划项目赠款的调查人员，以及4）协助 研究人员在准备动物的实验提出了在 这个项目的资助。最后，计划项目调查人员可以 咨询Averill博士（项目负责人）和Strawn博士（项目合作人）， 研究者）在需要时选择其他动物模型 根据本计划项目补助金的研究成果。 本计划项目中描述的实验将部分使用 半合子（mRen-2）27只转基因或Han SD大鼠。半合子（mRen-2）27 转基因大鼠是纯合（mRen-2）27雄性大鼠交配的后代 一只雌性SD大鼠这些半合子（mRen-2）27转基因 高血压大鼠被称为生产半合子动物 (TG*).据估计，与核心有关的动物设施 每年将向项目提供约325只Tg+和350只SD大鼠 在本计划拟议的5年期限内的项目调查员 项目更新。此外，核心将协调使用 每年将使用约145只SHR和140只WKY大鼠， 计划项目调查员。