Core--Tansgenic fish facility

该研究中心将促进在需要这些技术的不同项目中使用基因转移和鱼类转基因。该中心将为转基因构建提供建议，提供载体和报告基因，生产转基因鱼并对转基因鱼进行筛选。它还将提供处理和分析转基因鱼的指导。最终，随着含有萤火虫荧光素酶报告基因的新转基因品系的产生，它将为检测处理过的胚胎和幼虫是否受到毒物诱导提供指导。在这种情况下。本核心的具体目的是：1)为转基因载体的构建提供建议、载体、克隆调控元件和报告基因。对于那些不熟悉DNA工作的实验室，它将指导个人如何制造转基因结构和/或为实验室制造它们。2)通过DNA显微注射或伪型逆转录病毒载体感染制备转基因鱼。筛选有潜力的转基因奠基者的后代，鉴定表达转基因的后代，用于转基因品系的开发。指导个人（如有需要）对转基因品系进行检查、育种和转基因筛选。3)收集并冷冻转基因系的精子进行长期保存。4)进行育种，以便为治疗研究提供胚胎，并帮助对IRES转基因鱼进行荧光素酶分析。

This research core will facilitate the use of gene transfer and transgenes of fish in the different projects necessitating these technologies. The core will be setup to facilitate advice on transgenic construction, it will provide vectors and reporter genes, it will produce transgenic fish and screen the fish for the transgene. It will also provide instruction on handling and analyzing transgenic fish. Eventually, as new transgenic lines are produced that have firefly luciferase reporter genes, it will provide instructions on assaying the treated embryos and larvae for firefly induction by toxicants. In this context. the specific aims of this core will be: 1) To provide advice, vectors, cloned regulatory elements and reporter genes for the construction of transgenic vectors. For those laboratories not familiar with working with DNA, it will instruct individuals on how to make the transgenic constructs and/or make them for the laboratory. 2) To produce transgenic fish via DNA microinjection or pseudotyped retroviral vector infection. To screen the progeny of the potential transgenic founders to identify transgenic expressing progeny for the development of transgenic lines. To instruct individual, if desired, on the examination, breeding, and transgenic screening of the transgenic lines. 3) To collect and freeze down sperm from the transgenic lines for long- term storage. 4) To setup breedings so that embryos can be provided for treatment studies and to aid in the luciferase analysis of the IRES transgenic fish.